Literature DB >> 11250077

Monitoring promoter activity and protein localization in Mycobacterium spp. using green fluorescent protein.

S C Cowley1, Y Av-Gay.   

Abstract

Two green fluorescent protein (Gfp) fusion vectors were constructed for use in Mycobacterium spp. The first plasmid facilitates quantification of mycobacterial promoter activity. The second vector permits construction of translational fusions of mycobacterial proteins to Gfp in order to study subcellular localization including protein secretion. Using this translational fusion construct, we verify that a Gfp fusion to the putative secreted M. tuberculosis protein ChoD is translocated to the extracellular milieu when cloned and expressed in Mycobacterium smegmatis.

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Year:  2001        PMID： 11250077     DOI： 10.1016/s0378-1119(01)00336-5

Source DB:  PubMed          Journal:  Gene        ISSN： 0378-1119            Impact factor:   3.688


  20 in total

1.  Localization and functional analysis of PepI, the immunity peptide of Pep5-producing Staphylococcus epidermidis strain 5.

Authors:  Anja Hoffmann; Tanja Schneider; Ulrike Pag; Hans-Georg Sahl
Journal:  Appl Environ Microbiol       Date:  2004-06       Impact factor: 4.792

2.  Development of a liposome formulation of ethambutol.

Authors:  Theresa Wiens; Thomas Redelmeier; Yossef Av-Gay
Journal:  Antimicrob Agents Chemother       Date:  2004-05       Impact factor: 5.191

3.  Comparative analysis of mycobacterial truncated hemoglobin promoters and the groEL2 promoter in free-living and intracellular mycobacteria.

Authors:  Sunil V Joseph; G K Madhavilatha; R Ajay Kumar; Sathish Mundayoor
Journal:  Appl Environ Microbiol       Date:  2012-07-06       Impact factor: 4.792

4.  Madurastatin B3, a rare aziridine derivative from actinomycete Nocardiopsis sp. LS150010 with potent anti-tuberculosis activity.

Authors:  Xinjun Zhang; Hongtao He; Rong Ma; Zengchun Ji; Qi Wei; Huanqin Dai; Lixin Zhang; Fuhang Song
Journal:  J Ind Microbiol Biotechnol       Date:  2017-02-08       Impact factor: 3.346

5.  NR1D1 ameliorates Mycobacterium tuberculosis clearance through regulation of autophagy.

Authors:  Vemika Chandra; Ella Bhagyaraj; Ravikanth Nanduri; Nancy Ahuja; Pawan Gupta
Journal:  Autophagy       Date:  2015-11-02       Impact factor: 16.016

6.  Discovery of Mycobacterium tuberculosis α-1,4-glucan branching enzyme (GlgB) inhibitors by structure- and ligand-based virtual screening.

Authors:  Hedwin Kitdorlang Dkhar; Anupriya Gopalsamy; Saurabh Loharch; Amandeep Kaur; Isha Bhutani; Kanmani Saminathan; Ella Bhagyaraj; Vemika Chandra; Kunchithapadam Swaminathan; Pushpa Agrawal; Raman Parkesh; Pawan Gupta
Journal:  J Biol Chem       Date:  2014-11-10       Impact factor: 5.157

7.  Molecular characterization of the eis promoter of Mycobacterium tuberculosis.

Authors:  Esteban A Roberts; Amanda Clark; Sarah McBeth; Richard L Friedman
Journal:  J Bacteriol       Date:  2004-08       Impact factor: 3.490

8.  Human IL10 gene repression by Rev-erbα ameliorates Mycobacterium tuberculosis clearance.

Authors:  Vemika Chandra; Sahil Mahajan; Ankita Saini; Hedwin K Dkhar; Ravikanth Nanduri; Ella B Raj; Ashwani Kumar; Pawan Gupta
Journal:  J Biol Chem       Date:  2013-02-28       Impact factor: 5.157

9.  Kinome analysis of host response to mycobacterial infection: a novel technique in proteomics.

Authors:  Anne Lise K Hestvik; Zakaria Hmama; Yossef Av-Gay
Journal:  Infect Immun       Date:  2003-10       Impact factor: 3.441

10.  Truncated hemoglobin, HbN, is post-translationally modified in Mycobacterium tuberculosis and modulates host-pathogen interactions during intracellular infection.

Authors:  Swati Arya; Deepti Sethi; Sandeep Singh; Mangesh Dattu Hade; Vijender Singh; Preeti Raju; Sathi Babu Chodisetti; Deepshikha Verma; Grish C Varshney; Javed N Agrewala; Kanak L Dikshit
Journal:  J Biol Chem       Date:  2013-08-27       Impact factor: 5.157
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