| Literature DB >> 11234746 |
S Taheri1, J Gardiner, S Hafizi, K Murphy, C Dakin, L Seal, C Small, M Ghatei, S Bloom.
Abstract
The primary role of the orexins was originally believed to be appetite regulation, but is now believed to be the regulation of sleep, arousal and locomotor activity. Orexin A immunoreactivity (orexin A-IR) and prepro-orexin mRNA were measured in the CNS of obese and lean Zucker rats. There were no differences in orexin A-IR or prepro-orexin mRNA levels between obese and lean Zucker rats. The orexins are therefore unlikely to be important in this model of obesity. Levels of orexin A-IR and prepro-orexin mRNA were measured in the CNS of Wistar-Kyoto (WKY) rats, which are hypoactive and have abnormal sleep architecture. Compared to Wistar rats, WKY rats had significantly lower orexin A-IR (with differences of up to 100% in some brain regions) and prepro-orexin mRNA levels. These observations suggest that the sleep and activity phenotype of the WKY strain may be related to orexin deficiency and that this strain may be a useful model of partial orexin deficiency.Entities:
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Year: 2001 PMID： 11234746 DOI： 10.1097/00001756-200103050-00008
Source DB: PubMed Journal: Neuroreport ISSN： 0959-4965 Impact factor: 1.837