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Literature DB >> 11210937

Identification of a strong promoter of bacteriophage MB78 that interacts with a host coded factor and regulates the expression of a structural protein.

M A Zargar¹, D Chaturvedi, M Chakravort.

Abstract

A strong promoter of bacteriophage MB78 which controls the expression of a small structural protein of the phage has been identified and characterized. Analysis of its nucloetide sequence upstream of the translational start site revealed the presence of conserved -10 (TAATAT) and -35 (TTCTCCT) regions. It was observed that transcription initiates with thymidine residue, 86 nt upstream of the translational start site. Transcription seems to undergo alternate up and down regulation. This promoter is efficiently recognized by sigma 70 RNA polymerase and a host factor also binds to it. Binding of RNA polymerase is independent of binding of the host factor.

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Year: 2001 PMID： 11210937 DOI： 10.1023/a:1008178201795

Source DB: PubMed Journal: Virus Genes ISSN： 0920-8569 Impact factor: 2.332

15 in total

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

Authors: E M Southern
Journal: J Mol Biol Date: 1975-11-05 Impact factor: 5.469

2. Analysis of E. coli promoter sequences.

Authors: C B Harley; R P Reynolds
Journal: Nucleic Acids Res Date: 1987-03-11 Impact factor: 16.971

3. Use of minicells for bacteriophage-directed polypeptide synthesis.

Authors: J Reeve
Journal: Methods Enzymol Date: 1979 Impact factor: 1.600

4. Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate.

Authors: J P Chamberlain
Journal: Anal Biochem Date: 1979-09-15 Impact factor: 3.365

5. MB78, a virulent bacteriophage of Salmonella typhimurium.

Authors: A Joshi; J Z Siddiqi; G R Rao; M Chakravorty
Journal: J Virol Date: 1982-03 Impact factor: 5.103

6. Role of the sigma subunit of Escherichia coli RNA polymerase in initiation. I. Characterization of core enzyme open complexes.

Authors: U M Hansen; W R McClure
Journal: J Biol Chem Date: 1980-10-25 Impact factor: 5.157

1. Temperature sensitive mutation in the 38 kDa minor structural protein gene of phage MB78 interferes with phage morphogenesis.

Authors: Pinaki Datta; Prabhat Mallik; Amar N Ghosh; Maharani Chakravorthy
Journal: Virus Genes Date: 2005-03 Impact factor: 2.332

1 in total

Identification of a strong promoter of bacteriophage MB78 that interacts with a host coded factor and regulates the expression of a structural protein.

1. Detection of specific sequences among DNA fragments separated by gel electrophoresis.

2. Analysis of E. coli promoter sequences.

3. Use of minicells for bacteriophage-directed polypeptide synthesis.

4. Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate.

5. MB78, a virulent bacteriophage of Salmonella typhimurium.

6. Role of the sigma subunit of Escherichia coli RNA polymerase in initiation. I. Characterization of core enzyme open complexes.

7. Hybrid between temperate phage P22 and virulent phage MB78.

8. A membrane-filter technique for the detection of complementary DNA.

9. Plasmid vectors for the selection of promoters.

10. DNA sequencing with chain-terminating inhibitors.

1. Temperature sensitive mutation in the 38 kDa minor structural protein gene of phage MB78 interferes with phage morphogenesis.