OBJECTIVE: To determine the expression pattern of certain metalloproteinases (MMPs) known to be involved in the degradation of the extracellular matrix in cultured fibroblasts from the transversalis fascia (TF) of patients with inguinal hernia. SUMMARY BACKGROUND DATA: Inguinal hernia is a common pathology, the cause of which remains unknown. It is, however, clear that the TF is one of the anatomical structures that may impede the formation of hernias, and particularly the direct type of hernia. In previous studies the authors found enhanced MMP-2 expression in TF specimens in vivo. The persistence of increased expression in cultured fibroblasts might support the idea of a genetic defect as the cause for this pathology. METHODS: Fibroblasts from the TF of patients with direct and indirect inguinal hernia were cultured and compared with those obtained from control TF in terms of MMP (MMP-2 and MMP-9) expression. RESULTS: Significant active MMP-2 expression was shown by TF fibroblasts from young patients with direct hernias. These findings were confirmed by immunosorbent assay, immunoblotting, and zymography of the fibroblast culture media. No MMP-9 expression was detected. CONCLUSION: These results indicate that MMP-2 may be involved in the TF matrix degradative process in patients with direct hernia. The persistence of changes in MMP-2 levels in the cell cultures appears to suggest a genetic defect or irreversible change as the origin of this pathology rather than environmental factors, which may later participate in the development of the hernial process.
OBJECTIVE: To determine the expression pattern of certain metalloproteinases (MMPs) known to be involved in the degradation of the extracellular matrix in cultured fibroblasts from the transversalis fascia (TF) of patients with inguinal hernia. SUMMARY BACKGROUND DATA: Inguinal hernia is a common pathology, the cause of which remains unknown. It is, however, clear that the TF is one of the anatomical structures that may impede the formation of hernias, and particularly the direct type of hernia. In previous studies the authors found enhanced MMP-2 expression in TF specimens in vivo. The persistence of increased expression in cultured fibroblasts might support the idea of a genetic defect as the cause for this pathology. METHODS: Fibroblasts from the TF of patients with direct and indirect inguinal hernia were cultured and compared with those obtained from control TF in terms of MMP (MMP-2 and MMP-9) expression. RESULTS: Significant active MMP-2 expression was shown by TF fibroblasts from young patients with direct hernias. These findings were confirmed by immunosorbent assay, immunoblotting, and zymography of the fibroblast culture media. No MMP-9 expression was detected. CONCLUSION: These results indicate that MMP-2 may be involved in the TF matrix degradative process in patients with direct hernia. The persistence of changes in MMP-2 levels in the cell cultures appears to suggest a genetic defect or irreversible change as the origin of this pathology rather than environmental factors, which may later participate in the development of the hernial process.
Authors: Wojciech Szczęsny; Magdalena Kuligowska-Prusińska; Stanisław Dąbrowiecki; Jakub Szmytkowski; Adrian Reśliński; Maciej Słupski Journal: J Zhejiang Univ Sci B Date: 2018 Jan. Impact factor: 3.066
Authors: K-T von Trotha; J Grommes; N Butz; A Lambertz; C D Klink; U P Neumann; M Jacobs; M Binnebösel Journal: Hernia Date: 2017-04-08 Impact factor: 4.739
Authors: Lora Melman; Phillip R Chisholm; John A Curci; Batool Arif; Richard Pierce; Eric D Jenkins; L Michael Brunt; Christopher Eagon; Margaret Frisella; Kathleen Miller; Brent D Matthews Journal: Surg Endosc Date: 2010-01-07 Impact factor: 4.584
Authors: Zhi-Qiang Du; Xia Zhao; Natascha Vukasinovic; Fernanda Rodriguez; Archie C Clutter; Max F Rothschild Journal: PLoS One Date: 2009-03-16 Impact factor: 3.240