OBJECTIVE: The aim of this study was to investigate the effects of enamel matrix derivative (EMD) on gene expression of collagen alpha1 (I), osteocalcin, prostaglandin G/H synthase 2 (PGHS-2), interleukin-6, and insulin-like growth factor I in primary mouse osteoblasts. STUDY DESIGN: Primary osteoblasts were digested from 6- to 8-day-old mouse calvaria. Cells were divided into 4 groups and cultured for 24, 48, and 72 hours with a serum-free modified Eagle medium as negative control, modified Eagle medium with 25 microg/mL EMD, modified Eagle medium with 100 microg/mL EMD, and modified Eagle medium plus 10% fetal bovine serum as positive control. Gene expression was determined by Northern blot and reverse transcription polymerase chain reaction analysis. RESULT: EMD enhanced collagen I, interleukin-6, and PGHS-2 expression and did not stimulate the expression of osteocalcin and IGF-I. CONCLUSION: These results indicate that EMD might regulate certain gene expression during periodontal tissue regeneration.
OBJECTIVE: The aim of this study was to investigate the effects of enamel matrix derivative (EMD) on gene expression of collagen alpha1 (I), osteocalcin, prostaglandin G/H synthase 2 (PGHS-2), interleukin-6, and insulin-like growth factor I in primary mouse osteoblasts. STUDY DESIGN: Primary osteoblasts were digested from 6- to 8-day-old mouse calvaria. Cells were divided into 4 groups and cultured for 24, 48, and 72 hours with a serum-free modified Eagle medium as negative control, modified Eagle medium with 25 microg/mL EMD, modified Eagle medium with 100 microg/mL EMD, and modified Eagle medium plus 10% fetal bovine serum as positive control. Gene expression was determined by Northern blot and reverse transcription polymerase chain reaction analysis. RESULT: EMD enhanced collagen I, interleukin-6, and PGHS-2 expression and did not stimulate the expression of osteocalcin and IGF-I. CONCLUSION: These results indicate that EMD might regulate certain gene expression during periodontal tissue regeneration.
Authors: Richard J Miron; Erik Hedbom; Sabrina Ruggiero; Dieter D Bosshardt; Yufeng Zhang; Corinna Mauth; Anja C Gemperli; Tateyuki Iizuka; Daniel Buser; Anton Sculean Journal: PLoS One Date: 2011-08-15 Impact factor: 3.240
Authors: Daniela B Palioto; Thaisângela L Rodrigues; Julie T Marchesan; Márcio M Beloti; Paulo T de Oliveira; Adalberto L Rosa Journal: Head Face Med Date: 2011-07-18 Impact factor: 2.151