Literature DB >> 11160114

New host-vector system for Thermus spp. based on the malate dehydrogenase gene.

K J Kayser1, J J Kilbane.   

Abstract

A Thermus thermophilus HB27 strain was constructed in which the malate dehydrogenase (mdh) gene was deleted. The Deltamdh colonies are recognized by a small-colony phenotype. Wild-type phenotype is restored by transformation with Thermus plasmids or integration vector containing an intact mdh gene. The wild-type phenotype provides a positive selection tool for the introduction of plasmid DNA into Thermus spp., and because mdh levels can be readily quantified, this host-vector system is a convenient tool for monitoring gene expression.

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Year:  2001        PMID： 11160114      PMCID： PMC95068          DOI： 10.1128/JB.183.5.1792-1795.2001

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  11 in total

1.  Characterization of an operon encoding succinyl-CoA synthetase and malate dehydrogenase from Thermus flavus AT-62 and its expression in Escherichia coli.

Authors:  M Nishiyama; S Horinouchi; T Beppu
Journal:  Mol Gen Genet       Date:  1991-04

2.  Screening for thermostable mutant of kanamycin nucleotidyltransferase by the use of a transformation system for a thermophile, Bacillus stearothermophilus.

Authors:  M Matsumura; S Aiba
Journal:  J Biol Chem       Date:  1985-12-05       Impact factor: 5.157

Review 3.  [Comparative studies on biochemical properties of an extreme thermophile, Thermus thermophilus HB 8 (author's transl)].

Authors:  T Oshima
Journal:  Seikagaku       Date:  1974-10-25

4.  Development of Thermus-Escherichia shuttle vectors and their use for expression of the Clostridium thermocellum celA gene in Thermus thermophilus.

Authors:  I Lasa; M de Grado; M A de Pedro; J Berenguer
Journal:  J Bacteriol       Date:  1992-10       Impact factor: 3.490

5.  A plasmid vector for an extreme thermophile, Thermus thermophilus.

Authors:  Y Koyama; Y Arikawa; K Furukawa
Journal:  FEMS Microbiol Lett       Date:  1990-10       Impact factor: 2.742

6.  Thermus aquaticus gen. n. and sp. n., a nonsporulating extreme thermophile.

Authors:  T D Brock; H Freeze
Journal:  J Bacteriol       Date:  1969-04       Impact factor: 3.490

7.  Cloning and sequence analysis of the phenylalanyl-tRNA synthetase genes (pheST) from Thermus thermophilus.

Authors:  B Keller; P Kast; H Hennecke
Journal:  FEBS Lett       Date:  1992-04-13       Impact factor: 4.124

8.  Nucleotide sequence of the malate dehydrogenase gene of Thermus flavus and its mutation directing an increase in enzyme activity.

Authors:  M Nishiyama; N Matsubara; K Yamamoto; S Iijima; T Uozumi; T Beppu
Journal:  J Biol Chem       Date:  1986-10-25       Impact factor: 5.157

9.  Genetic transformation of the extreme thermophile Thermus thermophilus and of other Thermus spp.

Authors:  Y Koyama; T Hoshino; N Tomizuka; K Furukawa
Journal:  J Bacteriol       Date:  1986-04       Impact factor: 3.490

10.  An operon encoding aspartokinase and purine phosphoribosyltransferase in Thermus flavus.

Authors:  Makoto Nishiyama; Mutsuko Kukimoto; Teruhiko Beppu; Sueharu Horinouchi
Journal:  Microbiology (Reading)       Date:  1995-05       Impact factor: 2.777
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  4 in total

1.  Markerless Gene Deletion with Cytosine Deaminase in Thermus thermophilus Strain HB27.

Authors:  Lei Wang; Jana Hoffmann; Hildegard Watzlawick; Josef Altenbuchner
Journal:  Appl Environ Microbiol       Date:  2015-12-11       Impact factor: 4.792

2.  Heterologous gene expression in Thermus thermophilus: beta-galactosidase, dibenzothiophene monooxygenase, PNB carboxy esterase, 2-aminobiphenyl-2,3-diol dioxygenase, and chloramphenicol acetyl transferase.

Authors:  Ho-Shin Park; Kevin J Kayser; Jung-Ho Kwak; John J Kilbane
Journal:  J Ind Microbiol Biotechnol       Date:  2004-05-12       Impact factor: 3.346

3.  Expression of Vitreoscilla hemoglobin in Gordonia amarae enhances biosurfactant production.

Authors:  Ilhan Dogan; Krishna R Pagilla; Dale A Webster; Benjamin C Stark
Journal:  J Ind Microbiol Biotechnol       Date:  2006-02-21       Impact factor: 3.346

4.  Importance of the latex-clearing protein (Lcp) for poly(cis-1,4-isoprene) rubber cleavage in Streptomyces sp. K30.

Authors:  Meral Yikmis; Alexander Steinbüchel
Journal:  Microbiologyopen       Date:  2012-03       Impact factor: 3.139
  4 in total

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