Literature DB >> 11158150

Immunofluorescence method for detection of Ebola virus immunoglobulin g, using HeLa cells which express recombinant nucleoprotein.

M Saijo1, M Niikura, S Morikawa, I Kurane.   

Abstract

A novel recombinant baculovirus which expresses Ebola virus (EBO) nucleoprotein (NP) under the control of the cytomegalovirus immediate-early promoter was constructed. HeLa cells abortively infected with the baculovirus expressed EBO NP, and this was used as an immunofluorescent (IF) antigen to detect EBO immunoglobulin G (IgG) antibody. This IF method has high efficacy in detecting EBO IgG antibody in clinical specimens, indicating its usefulness in the diagnosis of EBO infections and seroepidemiological studies.

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Year:  2001        PMID： 11158150      PMCID： PMC87819          DOI： 10.1128/JCM.39.2.776-778.2001

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  20 in total

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Journal:  Virology       Date:  1989-05       Impact factor: 3.616

2.  Ebola haemorrhagic fever in Sudan, 1976. Report of a WHO/International Study Team.

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Journal:  Bull World Health Organ       Date:  1978       Impact factor: 9.408

3.  Ebola haemorrhagic fever in Zaire, 1976.

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Journal:  Bull World Health Organ       Date:  1978       Impact factor: 9.408

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Journal:  Lancet       Date:  1977-03-12       Impact factor: 79.321

5.  Linearization of baculovirus DNA enhances the recovery of recombinant virus expression vectors.

Authors:  P A Kitts; M D Ayres; R D Possee
Journal:  Nucleic Acids Res       Date:  1990-10-11       Impact factor: 16.971

6.  Epidemiology of Ebola (subtype Reston) virus in the Philippines, 1996.

Authors:  M E Miranda; T G Ksiazek; T J Retuya; A S Khan; A Sanchez; C F Fulhorst; P E Rollin; A B Calaor; D L Manalo; M C Roces; M M Dayrit; C J Peters
Journal:  J Infect Dis       Date:  1999-02       Impact factor: 5.226

7.  Baculovirus expression vectors: the requirements for high level expression of proteins, including glycoproteins.

Authors:  Y Matsuura; R D Possee; H A Overton; D H Bishop
Journal:  J Gen Virol       Date:  1987-05       Impact factor: 3.891

8.  Antibodies against haemorrhagic fever viruses in Kenya populations.

Authors:  B K Johnson; D Ocheng; A Gichogo; M Okiro; D Libondo; P M Tukei; M Ho; M Mugambi; G L Timms; M French
Journal:  Trans R Soc Trop Med Hyg       Date:  1983       Impact factor: 2.184

9.  Marburg, Ebola and Rift Valley Fever virus antibodies in East African primates.

Authors:  B K Johnson; L G Gitau; A Gichogo; P M Tukei; J G Else; M A Suleman; R Kimani; P D Sayer
Journal:  Trans R Soc Trop Med Hyg       Date:  1982       Impact factor: 2.184

10.  Preliminary report: isolation of Ebola virus from monkeys imported to USA.

Authors:  P B Jahrling; T W Geisbert; D W Dalgard; E D Johnson; T G Ksiazek; W C Hall; C J Peters
Journal:  Lancet       Date:  1990-03-03       Impact factor: 79.321
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  9 in total

1.  Antigen capture enzyme-linked immunosorbent assay for specific detection of Reston Ebola virus nucleoprotein.

Authors:  Tetsuro Ikegami; Masahiro Niikura; Masayuki Saijo; Mary E Miranda; Alan B Calaor; Marvin Hernandez; Luz P Acosta; Daria L Manalo; Ichiro Kurane; Yasuhiro Yoshikawa; Shigeru Morikawa
Journal:  Clin Diagn Lab Immunol       Date:  2003-07

Review 2.  Laboratory diagnostic systems for Ebola and Marburg hemorrhagic fevers developed with recombinant proteins.

Authors:  Masayuki Saijo; Masahiro Niikura; Tetsuro Ikegami; Ichiro Kurane; Takeshi Kurata; Shigeru Morikawa
Journal:  Clin Vaccine Immunol       Date:  2006-04

3.  Detection of Ebola viral antigen by enzyme-linked immunosorbent assay using a novel monoclonal antibody to nucleoprotein.

Authors:  M Niikura; T Ikegami; M Saijo; I Kurane; M E Miranda; S Morikawa
Journal:  J Clin Microbiol       Date:  2001-09       Impact factor: 5.948

4.  Novel immunofluorescence assay using recombinant nucleocapsid-spike fusion protein as antigen to detect antibodies against severe acute respiratory syndrome coronavirus.

Authors:  Qigai He; Ivanus Manopo; Liqun Lu; Bernard P Leung; Hiok Hee Chng; Ai Ee Ling; Li Lian Chee; Shzu-Wei Chan; Eng Eong Ooi; Yeo Lee Sin; Brenda Ang; Jimmy Kwang
Journal:  Clin Diagn Lab Immunol       Date:  2005-02

5.  Analysis of linear B-cell epitopes of the nucleoprotein of ebola virus that distinguish ebola virus subtypes.

Authors:  Masahiro Niikura; Tetsuro Ikegami; Masayuki Saijo; Takeshi Kurata; Ichiro Kurane; Shigeru Morikawa
Journal:  Clin Diagn Lab Immunol       Date:  2003-01

Review 6.  Towards detection and diagnosis of Ebola virus disease at point-of-care.

Authors:  Ajeet Kaushik; Sneham Tiwari; Rahul Dev Jayant; Aileen Marty; Madhavan Nair
Journal:  Biosens Bioelectron       Date:  2015-08-20       Impact factor: 10.618

7.  Rapid detection of viral antibodies based on multifunctional Staphylococcus aureus nanobioprobes.

Authors:  Jinjuan Qiao; Yunpeng Li; Cuihua Wei; Hang Yang; Junping Yu; Hongping Wei
Journal:  Enzyme Microb Technol       Date:  2016-09-20       Impact factor: 3.493

Review 8.  Serological assays based on recombinant viral proteins for the diagnosis of arenavirus hemorrhagic fevers.

Authors:  Shuetsu Fukushi; Hideki Tani; Tomoki Yoshikawa; Masayuki Saijo; Shigeru Morikawa
Journal:  Viruses       Date:  2012-10-12       Impact factor: 5.048

9.  Recurrent Ebolavirus disease in the Democratic Republic of Congo: update and challenges.

Authors:  Joseph Inungu; Kechi Iheduru-Anderson; Ossam J Odio
Journal:  AIMS Public Health       Date:  2019-11-20
  9 in total

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