| Literature DB >> 12853385 |
Tetsuro Ikegami1, Masahiro Niikura, Masayuki Saijo, Mary E Miranda, Alan B Calaor, Marvin Hernandez, Luz P Acosta, Daria L Manalo, Ichiro Kurane, Yasuhiro Yoshikawa, Shigeru Morikawa.
Abstract
Antigen capture enzyme-linked immunosorbent assay (ELISA) is one of the most useful methods to detect Ebola virus rapidly. We previously developed an antigen capture ELISA using a monoclonal antibody (MAb), 3-3D, which reacted not only to the nucleoprotein (NP) of Zaire Ebola virus (EBO-Z) but also to the NPs of Sudan (EBO-S) and Reston Ebola (EBO-R) viruses. In this study, we developed antigen capture ELISAs using two novel MAbs, Res2-6C8 and Res2-1D8, specific to the NP of EBO-R. Res2-6C8 and Res2-1D8 recognized epitopes consisting of 4 and 8 amino acid residues, respectively, near the C-terminal region of the EBO-R NP. The antigen capture ELISAs using these two MAbs detected the EBO-R NP in the tissues from EBO-R-infected cynomolgus macaques. The antigen capture ELISAs using Res2-6C8 and Res2-1D8 are useful for the rapid detection of the NP in EBO-R-infected cynomolgus macaques.Entities:
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Year: 2003 PMID： 12853385 PMCID： PMC164255 DOI： 10.1128/cdli.10.4.552-557.2003
Source DB: PubMed Journal: Clin Diagn Lab Immunol ISSN： 1071-412X