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Literature DB >> 11152392

Characteristics of the inhibitory effect of calmodulin on specific [125i]omega-conotoxin GVIA binding to crude membranes from chick brain.

S Ichida¹, J Abe, Y A Zhang, K Sugihara, K Imoto, T Wada, N Fujita, H Sohma.

Abstract

The characteristics of the inhibitory effect of calcium ion (Ca2+)/calmodulin (CaM) on specific [125I]-omega-conotoxin GVIA (125I-omega-CTX) binding and on the labeling of 125I-omega-CTX to crude membranes from chick brain were investigated. The inhibitory effect of Ca2+/CaM depended on the concentrations of free Ca2+ and CaM. The IC50 values for free Ca2+ and CaM were about 2.0 x 10(-8) M and 3.0 microg protein/ml, respectively. The inhibitory effect of Ca2+/CaM was attenuated by the CaM antagonists W-7, prenylamine and CaM-kinase II fragment (290-309), but not by the calcineurin inhibitor FK506. Ca2+/CaM also inhibited the labeling of a 135-kDa band (which was considered to be part of N-type Ca2+ channel alpha1 subunits) with 125I-omega-CTX using a cross-linker. These results suggest that Ca2+/CaM affects specific 125I-omega-CTX binding sites, probably N-type Ca2+ channel alpha1 subunits, in crude membranes from chick whole brain.

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Year: 2000 PMID： 11152392 DOI： 10.1023/a:1026674721542

Source DB: PubMed Journal: Neurochem Res ISSN： 0364-3190 Impact factor: 3.996

40 in total

1. Modulation of Ca2+ channels by G-protein beta gamma subunits.

Authors: S Herlitze; D E Garcia; K Mackie; B Hille; T Scheuer; W A Catterall
Journal: Nature Date: 1996-03-21 Impact factor: 49.962

Review 2. Calcium channels in vertebrate cells.

Authors: P Hess
Journal: Annu Rev Neurosci Date: 1990 Impact factor: 12.449

3. Evidence for distinct sites coupled to high affinity omega-conotoxin receptors in rat brain synaptic plasma membrane vesicles.

Authors: P Feigenbaum; M L Garcia; G J Kaczorowski
Journal: Biochem Biophys Res Commun Date: 1988-07-15 Impact factor: 3.575

4. Characteristics of [125I]omega-conotoxin labeling using bifunctional cross linker DSP in crude membranes from chick brain.

Authors: S Ichida; T Wada; T Akimoto; Y Kasamatsu; M Tahara; K Hasimoto
Journal: Neurochem Res Date: 1995-04 Impact factor: 3.996

5. Structural determinants of the blockade of N-type calcium channels by a peptide neurotoxin.

Authors: P T Ellinor; J F Zhang; W A Horne; R W Tsien
Journal: Nature Date: 1994-11-17 Impact factor: 49.962

6. Calmodulin activation of calcium-dependent sodium channels in excised membrane patches of Paramecium.

Authors: Y Saimi; K Y Ling
Journal: Science Date: 1990-09-21 Impact factor: 47.728

7. Purification and sequence of a presynaptic peptide toxin from Conus geographus venom.

Authors: B M Olivera; J M McIntosh; L J Cruz; F A Luque; W R Gray
Journal: Biochemistry Date: 1984-10-23 Impact factor: 3.162

8. Yeast calmodulin: structural and functional differences compared with vertebrate calmodulin.

Authors: Y Luan; I Matsuura; M Yazawa; T Nakamura; K Yagi
Journal: J Biochem Date: 1987-12 Impact factor: 3.387

9. Direct modulation by Ca(2+)-calmodulin of cyclic nucleotide-activated channel of rat olfactory receptor neurons.

Authors: T Y Chen; K W Yau
Journal: Nature Date: 1994-04-07 Impact factor: 49.962

10. Characteristics of specific 125I-omega-conotoxin GVIA binding in rat whole brain.

Authors: S Ichida; T Wada; M Sekiguchi; H Kishino; Y Okazaki; T Akimoto
Journal: Neurochem Res Date: 1993-11 Impact factor: 3.996

5 in total

1. Effects of calmodulin and Ca2+ channel blockers on omega-conotoxin GVIA binding to crude membranes from alpha1B subunit (Cav2.2) expressed BHK cells and mice brain lacking the alpha1B subunits.

Authors: Tetsuyuki Wada; Takashi Imanishi; Akinori Kawaguchi; Masayuki X Mori; Yasuo Mori; Keiji Imoto; Seiji Ichida
Journal: Neurochem Res Date: 2005-08 Impact factor: 3.996

2. Characteristics of omega-conotoxin GVI A and MVIIC binding to Cav 2.1 and Cav 2.2 channels captured by anti-Ca2+ channel peptide antibodies.

Authors: Seiji Ichida; Junichi Abe; Kuniyo Komoike; Takashi Imanishi; Tetsuyuki Wada; Takashi Masuko; Takeshi Minami
Journal: Neurochem Res Date: 2005-04 Impact factor: 3.996