Literature DB >> 11139637

RNA analysis by ion-pair reversed-phase high performance liquid chromatography.

A Azarani1, K H Hecker.   

Abstract

Ion-pair reversed-phase high performance liquid chromatography (IP RP HPLC) is presented as a new, superior method for the analysis of RNA. IP RP HPLC provides a fast and reliable alternative to classical methods of RNA analysis, including separation of different RNA species, quantification and purification. RNA is stable under the analysis conditions used; degradation of RNA during the analyses was not observed. The versatility of IP RP HPLC for RNA analysis is demonstrated. Components of an RNA ladder, ranging in size from 155 to 1770 nt, were resolved. RNA transcripts of up to 5219 nt were analyzed, their integrity determined and they were quantified and purified. Purification of mRNA from total RNA is described, separating mouse rRNA from poly(A)(+) mRNA. IP RP HPLC is also suitable for the separation and purification of DIG-labeled from unlabeled RNA. RNA purified by IP RP HPLC exhibits improved stability.

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Year:  2001        PMID： 11139637      PMCID： PMC29688          DOI： 10.1093/nar/29.2.e7

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  10 in total

1.  Allelic discrimination by denaturing high-performance liquid chromatography.

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Journal:  J Chromatogr B Biomed Sci Appl       Date:  2000-03-10

2.  Use of high-performance liquid chromatographic fractionation of large RNA molecules in the assay of group I intron ribozyme activity.

Authors:  D E Georgopoulos; M J Leibowitz
Journal:  J Chromatogr A       Date:  2000-01-28       Impact factor: 4.759

3.  Genotyping single nucleotide polymorphisms by primer extension and high performance liquid chromatography.

Authors:  B Hoogendoorn; M J Owen; P J Oefner; N Williams; J Austin; M C O'Donovan
Journal:  Hum Genet       Date:  1999-01       Impact factor: 4.132

4.  Optimization of cloning efficacy by pre-cloning DNA fragment analysis.

Authors:  K H Hecker; B Turpie; A Kuklin
Journal:  Biotechniques       Date:  1999-02       Impact factor: 1.993

5.  Blind analysis of denaturing high-performance liquid chromatography as a tool for mutation detection.

Authors:  M C O'Donovan; P J Oefner; S C Roberts; J Austin; B Hoogendoorn; C Guy; G Speight; M Upadhyaya; S S Sommer; P McGuffin
Journal:  Genomics       Date:  1998-08-15       Impact factor: 5.736

6.  Rapid selection of aminoacyl-tRNAs based on biotinylation of alpha-NH2 group of charged amino acids.

Authors:  J Pütz; J Wientges; M Sissler; R Giegé; C Florentz; A Schwienhorst
Journal:  Nucleic Acids Res       Date:  1997-05-01       Impact factor: 16.971

7.  Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.

Authors:  J C Alwine; D J Kemp; G R Stark
Journal:  Proc Natl Acad Sci U S A       Date:  1977-12       Impact factor: 11.205

8.  Use of PNA oligonucleotides for the in situ detection of Escherichia coli in water.

Authors:  A M Prescott; C R Fricker
Journal:  Mol Cell Probes       Date:  1999-08       Impact factor: 2.365

9.  Magnetic capture-hybridization method for purification and probing of mRNA for neutral protease of Bacillus cereus.

Authors:  H J Bach; A Hartmann; J T Trevors; J C Munch
Journal:  J Microbiol Methods       Date:  1999-08       Impact factor: 2.363

10.  High-resolution liquid chromatography of oligonucleotides on nonporous alkylated styrene-divinylbenzene copolymers.

Authors:  C G Huber; P J Oefner; G K Bonn
Journal:  Anal Biochem       Date:  1993-08-01       Impact factor: 3.365
  10 in total
  16 in total

1.  Rapid purification of RNA secondary structures.

Authors:  Stacy L Gelhaus; William R LaCourse; Nathan A Hagan; Gaya K Amarasinghe; Daniele Fabris
Journal:  Nucleic Acids Res       Date:  2003-11-01       Impact factor: 16.971

2.  Enrichment and analysis of RNA centered on ion pair reverse phase methodology.

Authors:  Mark J Dickman; David P Hornby
Journal:  RNA       Date:  2006-02-22       Impact factor: 4.942

3.  Rapid NMR screening of RNA secondary structure and binding.

Authors:  Christina Helmling; Sara Keyhani; Florian Sochor; Boris Fürtig; Martin Hengesbach; Harald Schwalbe
Journal:  J Biomol NMR       Date:  2015-07-19       Impact factor: 2.835

4.  Single-nucleotide resolution of RNAs up to 59 nucleotides by high-performance liquid chromatography.

Authors:  Zhen Huang; Sabarinath Jayaseelan; Jeffrey Hebert; Hyojung Seo; Li Niu
Journal:  Anal Biochem       Date:  2012-12-27       Impact factor: 3.365

Review 5.  Isotope labeling for studying RNA by solid-state NMR spectroscopy.

Authors:  Alexander Marchanka; Christoph Kreutz; Teresa Carlomagno
Journal:  J Biomol NMR       Date:  2018-04-12       Impact factor: 2.835

6.  Partitioning RNAs by length improves transcriptome reconstruction from short-read RNA-seq data.

Authors:  Francisca Rojas Ringeling; Shounak Chakraborty; Caroline Vissers; Derek Reiman; Akshay M Patel; Ki-Heon Lee; Ari Hong; Chan-Woo Park; Tim Reska; Julien Gagneur; Hyeshik Chang; Maria L Spletter; Ki-Jun Yoon; Guo-Li Ming; Hongjun Song; Stefan Canzar
Journal:  Nat Biotechnol       Date:  2022-01-10       Impact factor: 68.164

7.  A Platform for Discovery and Quantification of Modified Ribonucleosides in RNA: Application to Stress-Induced Reprogramming of tRNA Modifications.

Authors:  Weiling Maggie Cai; Yok Hian Chionh; Fabian Hia; Chen Gu; Stefanie Kellner; Megan E McBee; Chee Sheng Ng; Yan Ling Joy Pang; Erin G Prestwich; Kok Seong Lim; I Ramesh Babu; Thomas J Begley; Peter C Dedon
Journal:  Methods Enzymol       Date:  2015-07-17       Impact factor: 1.600

8.  A multidimensional platform for the purification of non-coding RNA species.

Authors:  Yok Hian Chionh; Chia-Hua Ho; Dumnoensun Pruksakorn; I Ramesh Babu; Chee Sheng Ng; Fabian Hia; Megan E McBee; Dan Su; Yan Ling Joy Pang; Chen Gu; Hongping Dong; Erin G Prestwich; Pei-Yong Shi; Peter Rainer Preiser; Sylvie Alonso; Peter C Dedon
Journal:  Nucleic Acids Res       Date:  2013-08-01       Impact factor: 16.971

Review 9.  Isotope labeling strategies for NMR studies of RNA.

Authors:  Kun Lu; Yasuyuki Miyazaki; Michael F Summers
Journal:  J Biomol NMR       Date:  2009-09-30       Impact factor: 2.835

10.  Key labeling technologies to tackle sizeable problems in RNA structural biology.

Authors:  Kwaku T Dayie
Journal:  Int J Mol Sci       Date:  2008-07-14       Impact factor: 6.208
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