| Literature DB >> 10445318 |
H J Bach1, A Hartmann, J T Trevors, J C Munch.
Abstract
A magnetic capture-hybridization method was assessed for the isolation of prokaryotic mRNA for the neutral protease of B. cereus from liquid culture. A biotin-labeled specific probe was hybridized to the mRNA transcripts and subsequently captured by streptavidin-coated paramagnetic beads. mRNA was detected by dot-blot hybridization with a ds DIG-labeled DNA-probe. The magnetic capture hybridization is a rapid and simple method and has a promising potential for gene expression studies in complex samples.Entities:
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Year: 1999 PMID： 10445318 DOI： 10.1016/s0167-7012(99)00066-4
Source DB: PubMed Journal: J Microbiol Methods ISSN： 0167-7012 Impact factor: 2.363