Literature DB >> 11133980

Identification of the outer membrane porin of Thermus thermophilus HB8: the channel-forming complex has an unusually high molecular mass and an extremely large single-channel conductance.

E Maier1, G Polleichtner, B Boeck, R Schinzel, R Benz.   

Abstract

The outer membrane of the thermophilic bacterium Thermus thermophilus was isolated using sucrose step gradient centrifugation. Its detergent extracts contained an ion-permeable channel with an extremely high single-channel conductance of 20 nS in 1 M KCl. The channel protein was purified by preparative sodium dodecyl sulfate (SDS)-polyacylamide gel electrophoresis. It has a high molecular mass of 185 kDa, and its channel-forming ability resists boiling in SDS for 10 min.

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Year:  2001        PMID： 11133980      PMCID： PMC94942          DOI： 10.1128/JB.183.2.800-803.2001

Source DB:  PubMed          Journal:  J Bacteriol        ISSN： 0021-9193            Impact factor:   3.490


  34 in total

1.  Biochemical identification and biophysical characterization of a channel-forming protein from Rhodococcus erythropolis.

Authors:  T Lichtinger; G Reiss; R Benz
Journal:  J Bacteriol       Date:  2000-02       Impact factor: 3.490

2.  Crystal structure of the outer membrane active transporter FepA from Escherichia coli.

Authors:  S K Buchanan; B S Smith; L Venkatramani; D Xia; L Esser; M Palnitkar; R Chakraborty; D van der Helm; J Deisenhofer
Journal:  Nat Struct Biol       Date:  1999-01

Review 3.  Molecular basis of bacterial outer membrane permeability.

Authors:  H Nikaido; M Vaara
Journal:  Microbiol Rev       Date:  1985-03

4.  Formation of large, ion-permeable membrane channels by the matrix protein (porin) of Escherichia coli.

Authors:  R Benz; K Janko; W Boos; P Läuger
Journal:  Biochim Biophys Acta       Date:  1978-08-17

5.  The ferrichrome-iron receptor of Escherichia coli K-12. Antigenicity of the fhuA protein.

Authors:  J W Coulton
Journal:  Biochim Biophys Acta       Date:  1982-07-16

6.  Proteins forming large channels from bacterial and mitochondrial outer membranes: porins and phage lambda receptor protein.

Authors:  H Nikaido
Journal:  Methods Enzymol       Date:  1983       Impact factor: 1.600

7.  Mechanism of sugar transport through the sugar-specific LamB channel of Escherichia coli outer membrane.

Authors:  R Benz; A Schmid; G H Vos-Scheperkeuter
Journal:  J Membr Biol       Date:  1987       Impact factor: 1.843

8.  Ion selectivity of gram-negative bacterial porins.

Authors:  R Benz; A Schmid; R E Hancock
Journal:  J Bacteriol       Date:  1985-05       Impact factor: 3.490

9.  Specificity of diffusion channels produced by lambda phage receptor protein of Escherichia coli.

Authors:  M Luckey; H Nikaido
Journal:  Proc Natl Acad Sci U S A       Date:  1980-01       Impact factor: 11.205

10.  Effect on solute size on diffusion rates through the transmembrane pores of the outer membrane of Escherichia coli.

Authors:  H Nikaido; E Y Rosenberg
Journal:  J Gen Physiol       Date:  1981-02       Impact factor: 4.086
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  10 in total

Review 1.  Molecular basis of bacterial outer membrane permeability revisited.

Authors:  Hiroshi Nikaido
Journal:  Microbiol Mol Biol Rev       Date:  2003-12       Impact factor: 11.056

Review 2.  DNA transport and natural transformation in mesophilic and thermophilic bacteria.

Authors:  Beate Averhoff
Journal:  J Bioenerg Biomembr       Date:  2004-02       Impact factor: 2.945

3.  Protein AQ_1862 from the hyperthermophilic bacterium Aquifex aeolicus is a porin and contains two conductance pathways of different selectivity.

Authors:  Ulrike Wedemeyer; Guohong Peng; Hartmut Michel; Klaus Hartung
Journal:  Biophys J       Date:  2007-06-22       Impact factor: 4.033

4.  Structure and function of PilQ, a secretin of the DNA transporter from the thermophilic bacterium Thermus thermophilus HB27.

Authors:  Janin Burkhardt; Janet Vonck; Beate Averhoff
Journal:  J Biol Chem       Date:  2011-02-01       Impact factor: 5.157

5.  Omp85(Tt) from Thermus thermophilus HB27: an ancestral type of the Omp85 protein family.

Authors:  Jutta Nesper; Alexander Brosig; Philippe Ringler; Geetika J Patel; Shirley A Müller; Jörg H Kleinschmidt; Winfried Boos; Kay Diederichs; Wolfram Welte
Journal:  J Bacteriol       Date:  2008-05-02       Impact factor: 3.490

6.  Elimination of channel-forming activity by insertional inactivation of the p13 gene in Borrelia burgdorferi.

Authors:  Yngve Ostberg; Marija Pinne; Roland Benz; Patricia Rosa; Sven Bergström
Journal:  J Bacteriol       Date:  2002-12       Impact factor: 3.490

7.  Expression, crystallization and preliminary X-ray analysis of an outer membrane protein from Thermus thermophilus HB27.

Authors:  Alexander Brosig; Jutta Nesper; Wolfram Welte; Kay Diederichs
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2008-05-23

8.  The outer membrane protein VhOmp of Vibrio harveyi: pore-forming properties in black lipid membranes.

Authors:  Albert Schulte; Sompong Ruamchan; Panida Khunkaewla; Wipa Suginta
Journal:  J Membr Biol       Date:  2009-08-13       Impact factor: 1.843

Review 9.  Thermus thermophilus as biological model.

Authors:  Felipe Cava; Aurelio Hidalgo; José Berenguer
Journal:  Extremophiles       Date:  2009-01-21       Impact factor: 2.395

Review 10.  Microbe-host interactions: structure and role of Gram-negative bacterial porins.

Authors:  Stefania Galdiero; Annarita Falanga; Marco Cantisani; Rossella Tarallo; Maria Elena Della Pepa; Virginia D'Oriano; Massimiliano Galdiero
Journal:  Curr Protein Pept Sci       Date:  2012-12       Impact factor: 3.272
  10 in total

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