PURPOSE: To investigate the role of oxidative stress in the development of diabetic retinopathy. METHODS: This study included 25 patients with diabetic retinopathy (group I), 34 patients with non-insulin-dependent diabetes mellitus without any angiopathy complications (group II) and 26 healthy subjects (group III). The serum malondialdehyde (MDA)-like metabolite levels as an index of lipid peroxidation, the erythrocyte glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and serum vitamin C levels of the patients and healthy subjects were measured. RESULTS: The mean serum concentration of MDA-like metabolites of patients in group I was 4.38 +/- 1.31 nmol/ml, in group II was 3.38 +/- 0.95 nmol/ml and in group III was 2.61 +/- 0.85 nmol/ml. There were significant differences between the groups (p = 0.001 for group I compared with group II, p = 0.0001 for group I compared with group III and p = 0.002 for group II compared with group III). There was a significant correlation between the serum lipid peroxidation concentrations and duration of the disease (r = 0.36, p = 0.047). The mean erythrocyte GSH-Px and SOD levels of group I were respectively 68.97 +/- 18.04 and 1597.78 +/- 296.46 U/g Hb, of group II were 64.30 +/- 19.26 and 1581.33 +/- 278.08 U/g Hb, and of group III were 65.52 +/- 17.58 and 1587.44 +/- 281.17 U/g Hb. There were no significant differences among the antioxidant enzyme levels in the three groups (p > 0.05). The mean serum vitamin C level in group I was 42.72 +/- 8.90 mumol/l, in group II was 49.26 +/- 11.52 mumol/l and in group III was 58.57 +/- 9.75 mumol/l. There were significant differences among the mean serum vitamin C levels of the three groups (p = 0.02 for group I versus group II p = 0.001 for group I versus group III and p = 0.002 for group II versus group III). CONCLUSIONS: Free radicals forming in diabetes mellitus and increasing over time may play a role in the development of diabetic retinopathy, which is an important complication of the disease.
PURPOSE: To investigate the role of oxidative stress in the development of diabetic retinopathy. METHODS: This study included 25 patients with diabetic retinopathy (group I), 34 patients with non-insulin-dependent diabetes mellitus without any angiopathy complications (group II) and 26 healthy subjects (group III). The serum malondialdehyde (MDA)-like metabolite levels as an index of lipid peroxidation, the erythrocyte glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and serum vitamin C levels of the patients and healthy subjects were measured. RESULTS: The mean serum concentration of MDA-like metabolites of patients in group I was 4.38 +/- 1.31 nmol/ml, in group II was 3.38 +/- 0.95 nmol/ml and in group III was 2.61 +/- 0.85 nmol/ml. There were significant differences between the groups (p = 0.001 for group I compared with group II, p = 0.0001 for group I compared with group III and p = 0.002 for group II compared with group III). There was a significant correlation between the serum lipid peroxidation concentrations and duration of the disease (r = 0.36, p = 0.047). The mean erythrocyte GSH-Px and SOD levels of group I were respectively 68.97 +/- 18.04 and 1597.78 +/- 296.46 U/g Hb, of group II were 64.30 +/- 19.26 and 1581.33 +/- 278.08 U/g Hb, and of group III were 65.52 +/- 17.58 and 1587.44 +/- 281.17 U/g Hb. There were no significant differences among the antioxidant enzyme levels in the three groups (p > 0.05). The mean serum vitamin C level in group I was 42.72 +/- 8.90 mumol/l, in group II was 49.26 +/- 11.52 mumol/l and in group III was 58.57 +/- 9.75 mumol/l. There were significant differences among the mean serum vitamin C levels of the three groups (p = 0.02 for group I versus group II p = 0.001 for group I versus group III and p = 0.002 for group II versus group III). CONCLUSIONS:Free radicals forming in diabetes mellitus and increasing over time may play a role in the development of diabetic retinopathy, which is an important complication of the disease.
Authors: Barbara Mysona; Ying Dun; Jennifer Duplantier; Vadivel Ganapathy; Sylvia B Smith Journal: Cell Tissue Res Date: 2009-01-21 Impact factor: 5.249
Authors: Maria F Lopes-Virella; Nathaniel L Baker; Kelly J Hunt; Timothy J Lyons; Alicia J Jenkins; Gabriel Virella Journal: Diabetes Care Date: 2012-04-17 Impact factor: 19.112