H Lalucque1, P Silar. 1. Institut de Génétique et Microbiologie, Université de Paris-Sud, France. lalucque@igmors.u-psud.fr
Abstract
BACKGROUND: To date, in eukaryotes, ribosomal protein expression is known to be regulated at the transcriptional and/or translational levels. But other forms of regulation may be possible. RESULTS: Here, we report the successful tagging of functional ribosomal particles with a S7-GFP chimaeric protein, making it possible to observe in vivo ribosome dynamics in the filamentous fungus Podospora anserina. Microscopic observations revealed a novel kind of ribosomal protein regulation during the passage between cell growth and stationary phases, with a transient accumulation of ribosomal proteins and/or ribosome subunits in the nucleus, possibly the nucleolus, being observed at the beginning of stationary phase. CONCLUSION: Nuclear sequestration can be another level of ribosomal protein regulation in eukaryotic cells. This may contribute to the regulation of cell growth and division.
BACKGROUND: To date, in eukaryotes, ribosomal protein expression is known to be regulated at the transcriptional and/or translational levels. But other forms of regulation may be possible. RESULTS: Here, we report the successful tagging of functional ribosomal particles with a S7-GFP chimaeric protein, making it possible to observe in vivo ribosome dynamics in the filamentous fungus Podospora anserina. Microscopic observations revealed a novel kind of ribosomal protein regulation during the passage between cell growth and stationary phases, with a transient accumulation of ribosomal proteins and/or ribosome subunits in the nucleus, possibly the nucleolus, being observed at the beginning of stationary phase. CONCLUSION: Nuclear sequestration can be another level of ribosomal protein regulation in eukaryotic cells. This may contribute to the regulation of cell growth and division.
Authors: W Shou; J H Seol; A Shevchenko; C Baskerville; D Moazed; Z W Chen; J Jang; A Shevchenko; H Charbonneau; R J Deshaies Journal: Cell Date: 1999-04-16 Impact factor: 41.582