Literature DB >> 11097931

Improved secretion of native human insulin-like growth factor 1 from gas1 mutant Saccharomyces cerevisiae cells.

M Vai1, L Brambilla, I Orlandi, N Rota, B M Ranzi, L Alberghina, D Porro.   

Abstract

We studied the secretion of recombinant human insulin-like growth factor 1 (rhIGF-1) from transformed yeast cells. The hIGF-1 gene was fused to the mating factor alpha prepro- leader sequence under the control of the constitutive ACT1 promoter. We found that the inactivation of the GAS1 gene in the host strain led to a supersecretory phenotype yielding a considerable increase, from 8 to 55 mg/liter, in rhIGF-1 production.

Entities:  

Mesh：

Substances：

Year:  2000        PMID： 11097931      PMCID： PMC92485          DOI： 10.1128/AEM.66.12.5477-5479.2000

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  28 in total

1.  Development of a pH-controlled fed-batch system for budding yeast.

Authors:  D Porro; E Martegani; A Tura; B M Ranzi
Journal:  Res Microbiol       Date:  1991-06       Impact factor: 3.992

2.  Increased expression in Escherichia coli of a synthetic gene encoding human somatomedin C after gene duplication and fusion.

Authors:  M F Schulz; G Buell; E Schmid; R Movva; G Selzer
Journal:  J Bacteriol       Date:  1987-12       Impact factor: 3.490

3.  Expression of a synthetic gene encoding human insulin-like growth factor I in cultured mouse fibroblasts.

Authors:  M L Bayne; M A Cascieri; B Kelder; J Applebaum; G Chicchi; J A Shapiro; F Pasleau; J J Kopchick
Journal:  Proc Natl Acad Sci U S A       Date:  1987-05       Impact factor: 11.205

4.  Expression, purification and characterization of recombinant human insulin-like growth factor I in yeast.

Authors:  M L Bayne; J Applebaum; G G Chicchi; N S Hayes; B G Green; M A Cascieri
Journal:  Gene       Date:  1988-06-30       Impact factor: 3.688

5.  The pro-region of the yeast prepro-alpha-factor is essential for membrane translocation of human insulin-like growth factor 1 in vivo.

Authors:  B Chaudhuri; K Steube; C Stephan
Journal:  Eur J Biochem       Date:  1992-06-15

6.  MCD4 encodes a conserved endoplasmic reticulum membrane protein essential for glycosylphosphatidylinositol anchor synthesis in yeast.

Authors:  E C Gaynor; G Mondésert; S J Grimme; S I Reed; P Orlean; S D Emr
Journal:  Mol Biol Cell       Date:  1999-03       Impact factor: 4.138

7.  Transcript accumulation of the GGP1 gene, encoding a yeast GPI-anchored glycoprotein, is inhibited during arrest in the G1 phase and during sporulation.

Authors:  L Popolo; P Cavadini; M Vai; L Alberghina
Journal:  Curr Genet       Date:  1993-11       Impact factor: 3.886

Review 8.  The Gas1 glycoprotein, a putative wall polymer cross-linker.

Authors:  L Popolo; M Vai
Journal:  Biochim Biophys Acta       Date:  1999-01-06

9.  A Lys27-to-Glu27 mutation in the human insulin-like growth factor-1 prevents disulfide linked dimerization and allows secretion of BiP when expressed in yeast.

Authors:  B Chaudhuri; S B Helliwell; J P Priestle
Journal:  FEBS Lett       Date:  1991-12-09       Impact factor: 4.124

10.  Physiological analysis of mutants indicates involvement of the Saccharomyces cerevisiae GPI-anchored protein gp115 in morphogenesis and cell separation.

Authors:  L Popolo; M Vai; E Gatti; S Porello; P Bonfante; R Balestrini; L Alberghina
Journal:  J Bacteriol       Date:  1993-04       Impact factor: 3.490
View more
  9 in total

Review 1.  Metabolic engineering of strains: from industrial-scale to lab-scale chemical production.

Authors:  Jie Sun; Hal S Alper
Journal:  J Ind Microbiol Biotechnol       Date:  2014-11-21       Impact factor: 3.346

Review 2.  Metabolic engineering of Saccharomyces cerevisiae: a key cell factory platform for future biorefineries.

Authors:  Kuk-Ki Hong; Jens Nielsen
Journal:  Cell Mol Life Sci       Date:  2012-03-03       Impact factor: 9.261

Review 3.  Recombinant protein production in yeasts.

Authors:  Danilo Porro; Michael Sauer; Paola Branduardi; Diethard Mattanovich
Journal:  Mol Biotechnol       Date:  2005-11       Impact factor: 2.695

4.  N-hypermannose glycosylation disruption enhances recombinant protein production by regulating secretory pathway and cell wall integrity in Saccharomyces cerevisiae.

Authors:  Hongting Tang; Shenghuan Wang; Jiajing Wang; Meihui Song; Mengyang Xu; Mengying Zhang; Yu Shen; Jin Hou; Xiaoming Bao
Journal:  Sci Rep       Date:  2016-05-09       Impact factor: 4.379

5.  Progress of Noncoding RNA Regulating the Growth and Development of Antler Tissue Research.

Authors:  Yipu Wang; Wei Hu
Journal:  Biomed Res Int       Date:  2022-07-19       Impact factor: 3.246

6.  Cloning of the Zygosaccharomyces bailii GAS1 homologue and effect of cell wall engineering on protein secretory phenotype.

Authors:  Simone Passolunghi; Luca Riboldi; Laura Dato; Danilo Porro; Paola Branduardi
Journal:  Microb Cell Fact       Date:  2010-01-26       Impact factor: 5.328

7.  Random and direct mutagenesis to enhance protein secretion in Ashbya gossypii.

Authors:  Orquídea Ribeiro; Frederico Magalhães; Tatiana Q Aguiar; Marilyn G Wiebe; Merja Penttilä; Lucília Domingues
Journal:  Bioengineered       Date:  2013-04-15       Impact factor: 3.269

8.  Recombinant production of mecasermin in E. coli expression system.

Authors:  S Jafari; V Babaeipour; H A Eslampanah Seyedi; M Rahaie; M R Mofid; L Haddad; M M Namvaran; J Fallah
Journal:  Res Pharm Sci       Date:  2014 Nov-Dec

9.  Production of secretory cutinase by recombinant Saccharomyces cerevisiae protoplasts.

Authors:  Hideki Aoyagi; Yoichi Katakura; Akio Iwasaki
Journal:  Springerplus       Date:  2016-02-24
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.