Literature DB >> 11090142

Analysis of individual human trigeminal ganglia for latent herpes simplex virus type 1 and varicella-zoster virus nucleic acids using real-time PCR.

R J Cohrs1, J Randall, J Smith, D H Gilden, C Dabrowski, H van Der Keyl, R Tal-Singer.   

Abstract

Herpes simplex virus type 1 (HSV-1) and varicella-zoster virus (VZV) establish latent infections in the peripheral nervous system following primary infection. During latency both virus genomes exhibit limited transcription, with the HSV-1 LATs and at least four VZV transcripts consistently detected in latently infected human ganglia. In this study we used real-time PCR quantitation to determine the viral DNA copy number in individual trigeminal ganglia (TG) from 17 subjects. The number of HSV-1 genomes was not significantly different between the left and right TG from the same individual and varied per subject from 42.9 to 677.9 copies per 100 ng of DNA. The number of VZV genomes was also not significantly different between left and right TG from the same individual and varied per subject from 37.0 to 3,560.5 copies per 100 ng of DNA. HSV-1 LAT transcripts were consistently detected in ganglia containing latent HSV-1 and varied in relative expression by >500-fold. Of the three VZV transcripts analyzed, only transcripts mapping to gene 63 were consistently detected in latently infected ganglia and varied in relative expression by >2,000-fold. Thus, it appears that, similar to LAT transcription in HSV-1 latently infected ganglia, VZV gene 63 transcription is a hallmark of VZV latency.

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Year:  2000        PMID: 11090142      PMCID: PMC112425          DOI: 10.1128/jvi.74.24.11464-11471.2000

Source DB:  PubMed          Journal:  J Virol        ISSN: 0022-538X            Impact factor:   5.103


  37 in total

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Authors:  R Mahalingam; P G Kennedy; D H Gilden
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Review 2.  Neurologic complications of the reactivation of varicella-zoster virus.

Authors:  D H Gilden; B K Kleinschmidt-DeMasters; J J LaGuardia; R Mahalingam; R J Cohrs
Journal:  N Engl J Med       Date:  2000-03-02       Impact factor: 91.245

Review 3.  Repression of viral transcription during herpes simplex virus latency.

Authors:  C M Preston
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4.  Numbers of neurons and non-neuronal cells in human trigeminal ganglia.

Authors:  J J LaGuardia; R J Cohrs; D H Gilden
Journal:  Neurol Res       Date:  2000-09       Impact factor: 2.448

5.  The latent herpes simplex virus type 1 genome copy number in individual neurons is virus strain specific and correlates with reactivation.

Authors:  N M Sawtell; D K Poon; C S Tansky; R L Thompson
Journal:  J Virol       Date:  1998-07       Impact factor: 5.103

6.  Use of differential display reverse transcription-PCR to reveal cellular changes during stimuli that result in herpes simplex virus type 1 reactivation from latency: upregulation of immediate-early cellular response genes TIS7, interferon, and interferon regulatory factor-1.

Authors:  R Tal-Singer; W Podrzucki; T M Lasner; A Skokotas; J J Leary; N W Fraser; S L Berger
Journal:  J Virol       Date:  1998-02       Impact factor: 5.103

7.  Gene expression during reactivation of herpes simplex virus type 1 from latency in the peripheral nervous system is different from that during lytic infection of tissue cultures.

Authors:  R Tal-Singer; T M Lasner; W Podrzucki; A Skokotas; J J Leary; S L Berger; N W Fraser
Journal:  J Virol       Date:  1997-07       Impact factor: 5.103

8.  Quantitation of latent varicella-zoster virus and herpes simplex virus genomes in human trigeminal ganglia.

Authors:  S R Pevenstein; R K Williams; D McChesney; E K Mont; J E Smialek; S E Straus
Journal:  J Virol       Date:  1999-12       Impact factor: 5.103

Review 9.  Molecular circuitry regulating herpes simplex virus type 1 latency in neurons.

Authors:  S Millhouse; B Wigdahl
Journal:  J Neurovirol       Date:  2000-02       Impact factor: 2.643

10.  Latent varicella-zoster virus is located predominantly in neurons in human trigeminal ganglia.

Authors:  P G Kennedy; E Grinfeld; J W Gow
Journal:  Proc Natl Acad Sci U S A       Date:  1998-04-14       Impact factor: 11.205

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  83 in total

1.  Neutralizing anti-gH antibody of Varicella-zoster virus modulates distribution of gH and induces gene regulation, mimicking latency.

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Journal:  J Virol       Date:  2011-06-01       Impact factor: 5.103

2.  The relationship of herpes simplex virus latency associated transcript expression to genome copy number: a quantitative study using laser capture microdissection.

Authors:  Xiao-Ping Chen; Marina Mata; Mary Kelley; Joseph C Glorioso; David J Fink
Journal:  J Neurovirol       Date:  2002-06       Impact factor: 2.643

Review 3.  Neurovirological methods and their applications.

Authors:  P G E Kennedy
Journal:  J Neurol Neurosurg Psychiatry       Date:  2003-08       Impact factor: 10.154

4.  Burning mouth syndrome due to herpes simplex virus type 1.

Authors:  Maria A Nagel; Alexander Choe; Igor Traktinskiy; Don Gilden
Journal:  BMJ Case Rep       Date:  2015-04-01

5.  Herpes Simplex Virus 1 Replication, Ocular Disease, and Reactivations from Latency Are Restricted Unilaterally after Inoculation of Virus into the Lip.

Authors:  Nolwenn Poccardi; Antoine Rousseau; Oscar Haigh; Julie Takissian; Thierry Naas; Claire Deback; Louise Trouillaud; Mohammad Issa; Simon Roubille; Franceline Juillard; Stacey Efstathiou; Patrick Lomonte; Marc Labetoulle
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6.  Varicella-zoster virus ORF47 protein kinase, which is required for replication in human T cells, and ORF66 protein kinase, which is expressed during latency, are dispensable for establishment of latency.

Authors:  Hitoshi Sato; Lesley Pesnicak; Jeffrey I Cohen
Journal:  J Virol       Date:  2003-10       Impact factor: 5.103

7.  Nuclear import of the varicella-zoster virus latency-associated protein ORF63 in primary neurons requires expression of the lytic protein ORF61 and occurs in a proteasome-dependent manner.

Authors:  Matthew S Walters; Christos A Kyratsous; Shilin Wan; Saul Silverstein
Journal:  J Virol       Date:  2008-06-18       Impact factor: 5.103

8.  Identification of phosphorylated residues on varicella-zoster virus immediate-early protein ORF63.

Authors:  Niklaus H Mueller; Matthew S Walters; Roland A Marcus; Laurie L Graf; Jessica Prenni; Don Gilden; Saul J Silverstein; Randall J Cohrs
Journal:  J Gen Virol       Date:  2010-01-20       Impact factor: 3.891

9.  Detection and genotyping of varicella-zoster virus by TaqMan allelic discrimination real-time PCR.

Authors:  Paul A Campsall; Nicholas H C Au; Julie S Prendiville; David P Speert; Rusung Tan; Eva E Thomas
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10.  Downregulation of varicella-zoster virus (VZV) immediate-early ORF62 transcription by VZV ORF63 correlates with virus replication in vitro and with latency.

Authors:  Susan E Hoover; Randall J Cohrs; Zoila G Rangel; Donald H Gilden; Peter Munson; Jeffrey I Cohen
Journal:  J Virol       Date:  2006-04       Impact factor: 5.103

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