Literature DB >> 11080295

Purification and characterization of two voltage-dependent anion channel isoforms from plant seeds.

H Abrecht1, R Wattiez, J M Ruysschaert, F Homblé.   

Abstract

Mitochondria were isolated from imbibed seeds of lentil (Lens culinaris) and Phaseolus vulgaris. We copurified two voltage-dependent anion channel from detergent solubilized mitochondria in a single purification step using hydroxyapatite. The two isoforms from P. vulgaris were separated by chromatofocusing chromatography in 4 M urea without any loss of channel activity. Channel activity of each isoform was characterized upon reconstitution into diphytanoyl phosphatidylcholine planar lipid bilayers. Both isoforms form large conductance channels that are slightly anion selective and display cation selective substates.

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Year:  2000        PMID： 11080295      PMCID： PMC59217          DOI： 10.1104/pp.124.3.1181

Source DB:  PubMed          Journal:  Plant Physiol        ISSN： 0032-0889            Impact factor:   8.340


  40 in total

1.  Bcl-2 family proteins regulate the release of apoptogenic cytochrome c by the mitochondrial channel VDAC.

Authors:  S Shimizu; M Narita; Y Tsujimoto
Journal:  Nature       Date:  1999-06-03       Impact factor: 49.962

2.  Regulation of metabolite flux through voltage-gating of VDAC channels.

Authors:  T Hodge; M Colombini
Journal:  J Membr Biol       Date:  1997-06-01       Impact factor: 1.843

3.  Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis.

Authors:  D W Cleveland; S G Fischer; M W Kirschner; U K Laemmli
Journal:  J Biol Chem       Date:  1977-02-10       Impact factor: 5.157

4.  Cleavage of structural proteins during the assembly of the head of bacteriophage T4.

Authors:  U K Laemmli
Journal:  Nature       Date:  1970-08-15       Impact factor: 49.962

5.  Biochemical, molecular, and functional characterization of porin isoforms from potato mitochondria.

Authors:  L Heins; H Mentzel; A Schmid; R Benz; U K Schmitz
Journal:  J Biol Chem       Date:  1994-10-21       Impact factor: 5.157

6.  Reconstitution in planar lipid bilayers of a voltage-dependent anion-selective channel obtained from paramecium mitochondria.

Authors:  S J Schein; M Colombini; A Finkelstein
Journal:  J Membr Biol       Date:  1976-12-28       Impact factor: 1.843

Review 7.  Porin interaction with hexokinase and glycerol kinase: metabolic microcompartmentation at the outer mitochondrial membrane.

Authors:  V Adams; L Griffin; J Towbin; B Gelb; K Worley; E R McCabe
Journal:  Biochem Med Metab Biol       Date:  1991-06

8.  Purification and Characterization of Porin from Corn (Zea mays L.) Mitochondria.

Authors:  J. A. Aljamal; G. Genchi; V. De Pinto; L. Stefanizzi; A. De Santis; R. Benz; F. Palmieri
Journal:  Plant Physiol       Date:  1993-06       Impact factor: 8.340

9.  Identification of porin as a binding site for MAP2.

Authors:  M Lindén; G Karlsson
Journal:  Biochem Biophys Res Commun       Date:  1996-01-26       Impact factor: 3.575

10.  Alkylation of cysteine with acrylamide for protein sequence analysis.

Authors:  D C Brune
Journal:  Anal Biochem       Date:  1992-12       Impact factor: 3.365
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  7 in total

1.  Intracellular localization of VDAC proteins in plants.

Authors:  Cathrin Clausen; Iryna Ilkavets; Rowena Thomson; Katrin Philippar; Aleksandar Vojta; Torsten Möhlmann; Ekkehard Neuhaus; Hrvoje Fulgosi; Jürgen Soll
Journal:  Planta       Date:  2004-07-16       Impact factor: 4.116

2.  Modulation of plant mitochondrial VDAC by phytosterols.

Authors:  Lamia Mlayeh; Sunita Chatkaew; Marc Léonetti; Fabrice Homblé
Journal:  Biophys J       Date:  2010-10-06       Impact factor: 4.033

3.  Concentration dependent ion selectivity in VDAC: a molecular dynamics simulation study.

Authors:  Eva-Maria Krammer; Fabrice Homblé; Martine Prévost
Journal:  PLoS One       Date:  2011-12-02       Impact factor: 3.240

4.  Dual mechanism of ion permeation through VDAC revealed with inorganic phosphate ions and phosphate metabolites.

Authors:  Eva-Maria Krammer; Giang Thi Vu; Fabrice Homblé; Martine Prévost
Journal:  PLoS One       Date:  2015-04-10       Impact factor: 3.240

Review 5.  VDACs Post-Translational Modifications Discovery by Mass Spectrometry: Impact on Their Hub Function.

Authors:  Maria Gaetana Giovanna Pittalà; Stefano Conti Nibali; Simona Reina; Vincenzo Cunsolo; Antonella Di Francesco; Vito De Pinto; Angela Messina; Salvatore Foti; Rosaria Saletti
Journal:  Int J Mol Sci       Date:  2021-11-27       Impact factor: 5.923

Review 6.  Calcium Flux across Plant Mitochondrial Membranes: Possible Molecular Players.

Authors:  Luca Carraretto; Vanessa Checchetto; Sara De Bortoli; Elide Formentin; Alex Costa; Ildikó Szabó; Enrico Teardo
Journal:  Front Plant Sci       Date:  2016-03-31       Impact factor: 5.753

7.  The Open State Selectivity of the Bean Seed VDAC Depends on Stigmasterol and Ion Concentration.

Authors:  Hayet Saidani; Marc Léonetti; Hanna Kmita; Fabrice Homblé
Journal:  Int J Mol Sci       Date:  2021-03-16       Impact factor: 5.923
  7 in total

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