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Literature DB >> 1107809

Genetic consequences of transfection with heterduplex bacteriophage lambda DNA.

Abstract

The role of rectification of heteroduplex heterozygotes in the formation of recombinant genotypes involving closely linked markers has been examined. Heteroduplex molecules of bacteriophage lambda DNA, heterozygous at several alleles, have been constructed and the genetic composition of phage present in infective centers derived by transfection with such molecules has been determined. Allele loss and concomitant recombinant formation is frequent, and appears to reflect marker specificity as well as specificities imposed by whether or not the transfection recipient is permissive or nonpermissive for DNA duplication of the transfecting genome. The observations support the proposal that many, perhaps most, of the events involving separation of closely linked markers occur by rectification of non-recombinant heterozygotes.

Entities: Species

Mesh：

Substances：
DNA, Viral

Year: 1975 PMID： 1107809 DOI： 10.1007/bf00267681

Source DB: PubMed Journal: Mol Gen Genet ISSN： 0026-8925

15 in total

7. A fine structure map of spontaneous and induced mutations in the lambda repressor gene, including insertions of IS elements.

Authors: M Lieb
Journal: Mol Gen Genet Date: 1981

Genetic consequences of transfection with heterduplex bacteriophage lambda DNA.

1. Mismatch repair in heteroduplex DNA.

2. Genetic heterozygosity in unreplicated bacteriophage lambda recombinants.

3. ON THE MECHANISM OF GENETIC RECOMBINATION BETWEEN DNA MOLECULES.

4. MOLECULAR MECHANISMS OF GENETIC RECOMBINATION IN BACTERIOPHAGE. II. JOINING OF PARENTAL DNA MOLECULES OF PHAGE T4.

5. Heterozygosis and recombination of bacteriophage.

6. On the molecular basis of high negative interference.

7. On the physical structure of lambda recombinant DNA.

8. Position of branch points in replicating lambda DNA.

9. One way to do experiments on gene conversion? Transfection with heteroduplex SPP1 DNA.

10. On the mechanism of integration of transforming deoxyribonucleate.

1. Repair tracts in mismatched DNA heteroduplexes.

2. Fate of mismatched base-pair regions in polyoma heteroduplex DNA during infection of mouse cells.

3. Repair of single-stranded DNA nicks, gaps, and loops in mammalian cells.

4. Cloning of an immunoglobulin variable region gene from mouse embryo.

5. Efficient correction of mismatched bases in plasmid heteroduplexes injected into cultured mammalian cell nuclei.

6. Specific mismatch correction in bacteriophage lambda crosses by very short patch repair.

7. A fine structure map of spontaneous and induced mutations in the lambda repressor gene, including insertions of IS elements.

Review 8. Mechanisms in E. coli and Human Mismatch Repair (Nobel Lecture).

9. Correction of complex heteroduplexes made of mouse H-2 gene sequences in Escherichia coli K-12.

10. Induced mutagenesis in dam- mutants of Escherichia coli: a role for 6-methyladenine residues in mutation avoidance.