Literature DB >> 11056400

Efficient cloning and engineering of giant DNAs in a novel Bacillus subtilis genome vector.

M Itaya1, T Nagata, T Shiroishi, K Fujita, K Tsuge.   

Abstract

The Genome of Bacillus subtilis 168 was used for cloning and engineering of large-sized DNAs. A mouse genomic DNA of approximately 120 kb was cloned into a locus of the B. subtilis genome by ordered assembly of 20- to 50-kb mouse DNA segments. Cloned mouse DNA, maintained stably, was engineered through B. subtilis transformation and recombination. Creation of an I-PpoI recognition sequence at both ends of the insert facilitated its isolation by pulsed field gel electrophoresis. The basic concept of genome vector technology is suited to the handling of DNAs larger than 100 kb.

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Year:  2000        PMID： 11056400     DOI： 10.1093/oxfordjournals.jbchem.a022825

Source DB:  PubMed          Journal:  J Biochem        ISSN： 0021-924X            Impact factor:   3.387


  16 in total

Review 1.  Manipulating corynebacteria, from individual genes to chromosomes.

Authors:  Alain A Vertès; Masayuki Inui; Hideaki Yukawa
Journal:  Appl Environ Microbiol       Date:  2005-12       Impact factor: 4.792

2.  Combining two genomes in one cell: stable cloning of the Synechocystis PCC6803 genome in the Bacillus subtilis 168 genome.

Authors:  Mitsuhiro Itaya; Kenji Tsuge; Maki Koizumi; Kyoko Fujita
Journal:  Proc Natl Acad Sci U S A       Date:  2005-10-18       Impact factor: 11.205

3.  Horizontal transfer of iturin A operon, itu, to Bacillus subtilis 168 and conversion into an iturin A producer.

Authors:  Kenji Tsuge; Satoka Inoue; Takashi Ano; Mitsuhiro Itaya; Makoto Shoda
Journal:  Antimicrob Agents Chemother       Date:  2005-11       Impact factor: 5.191

4.  Metabolic engineering of carotenoid biosynthesis in Escherichia coli by ordered gene assembly in Bacillus subtilis.

Authors:  Tomoko Nishizaki; Kenji Tsuge; Mitsuhiro Itaya; Nobuhide Doi; Hiroshi Yanagawa
Journal:  Appl Environ Microbiol       Date:  2006-12-28       Impact factor: 4.792

Review 5.  Strategies for cloning and manipulating natural and synthetic chromosomes.

Authors:  Bogumil J Karas; Yo Suzuki; Philip D Weyman
Journal:  Chromosome Res       Date:  2015-02       Impact factor: 5.239

6.  Integration of stable extracellular DNA released from Escherichia coli into the Bacillus subtilis genome vector by culture mix method.

Authors:  Mitsuhiro Itaya; Shinya Kaneko
Journal:  Nucleic Acids Res       Date:  2010-03-22       Impact factor: 16.971

7.  One step assembly of multiple DNA fragments with a designed order and orientation in Bacillus subtilis plasmid.

Authors:  Kenji Tsuge; Kuniko Matsui; Mitsuhiro Itaya
Journal:  Nucleic Acids Res       Date:  2003-11-01       Impact factor: 16.971

8.  Targeted isolation of a designated region of the Bacillus subtilis genome by recombinational transfer.

Authors:  Satoshi Tomita; Kenji Tsuge; Yo Kikuchi; Mitsuhiro Itaya
Journal:  Appl Environ Microbiol       Date:  2004-04       Impact factor: 4.792

Review 9.  Direct Capture Technologies for Genomics-Guided Discovery of Natural Products.

Authors:  Andrew N Chan; Kevin C Santa Maria; Bo Li
Journal:  Curr Top Med Chem       Date:  2016       Impact factor: 3.295

10.  Conversion of sub-megasized DNA to desired structures using a novel Bacillus subtilis genome vector.

Authors:  Shinya Kaneko; Kenji Tsuge; Takashi Takeuchi; Mitsuhiro Itaya
Journal:  Nucleic Acids Res       Date:  2003-09-15       Impact factor: 16.971
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