BACKGROUND AND OBJECTIVES: The expression of HLA-C on the surface of platelets is rarely studied due to the lack of proper alloantisera. We addressed this question using an IgM human monoclonal antibody directed against HLA-Cw1 (VP6G3). MATERIAL AND METHODS: Both flow cytometry and complement dependent cytotoxicity studies were used in the current analysis. RESULTS: The expression of the HLA-Cw1 antigen on platelets is lower than on peripheral blood lymphocytes as shown by flow cytometry. Variation in expression levels between individuals is also observed. Using this antibody in a complement-dependent cytotoxicity assay, we did not observe lysis using platelets as targets, whereas peripheral blood lymphocytes of the same blood donors were adequately lysed. CONCLUSIONS: These results confirm that platelets indeed express HLA-C. Furthermore, the results support the insignificant role of HLA-C in immunological platelet refractoriness. Copyright 2000 S. Karger AG, Basel
BACKGROUND AND OBJECTIVES: The expression of HLA-C on the surface of platelets is rarely studied due to the lack of proper alloantisera. We addressed this question using an IgM human monoclonal antibody directed against HLA-Cw1 (VP6G3). MATERIAL AND METHODS: Both flow cytometry and complement dependent cytotoxicity studies were used in the current analysis. RESULTS: The expression of the HLA-Cw1 antigen on platelets is lower than on peripheral blood lymphocytes as shown by flow cytometry. Variation in expression levels between individuals is also observed. Using this antibody in a complement-dependent cytotoxicity assay, we did not observe lysis using platelets as targets, whereas peripheral blood lymphocytes of the same blood donors were adequately lysed. CONCLUSIONS: These results confirm that platelets indeed express HLA-C. Furthermore, the results support the insignificant role of HLA-C in immunological platelet refractoriness. Copyright 2000 S. Karger AG, Basel
Authors: Robert O Endres; Steven H Kleinman; Danielle M Carrick; Whitney R Steele; David J Wright; Philip J Norris; Darrell Triulzi; Ram Kakaiya; Michael P Busch Journal: Transfusion Date: 2010-02-11 Impact factor: 3.157
Authors: Cynthia S M Kramer; Marry E I Franke-van Dijk; Ashley J Priddey; Tamás Pongrácz; Elena Gnudi; Helena Car; Gonca E Karahan; Els van Beelen; Chalana C C Zilvold-van den Oever; Hendrik J Rademaker; Noortje de Haan; Manfred Wuhrer; Vasilis Kosmoliaptsis; Paul W H I Parren; Arend Mulder; Dave L Roelen; Frans H J Claas; Sebastiaan Heidt Journal: HLA Date: 2019-08-25 Impact factor: 4.513