Literature DB >> 11049752

Characterization of a recombinant murine 18.5-kDa myelin basic protein.

I R Bates1, P Matharu, N Ishiyama, D Rochon, D D Wood, E Polverini, M A Moscarello, N J Viner, G Harauz.   

Abstract

A recombinant hexahistidine-tagged 18.5-kDa isoform of murine myelin basic protein has been characterized biochemically and immunogenically, by mass spectrometry, by circular dichroism under various conditions (in aqueous solution, with monosialoganglioside G(M1), and in 89% 2-propanol), and by transmission electron microscopy. The preparations of this protein indicated a high degree of purity and homogeneity, with no significant posttranslational modifications. Circular dichroic spectra showed that this preparation had the same degree of secondary structure as the natural bovine 18.5-kDa isoform of myelin basic protein. Incubation of the recombinant protein with lipid monolayers containing a nickel-chelating lipid resulted in the formation of fibrous assemblies that formed paracrystals of spacings 4.8 nm between fibers and 3-4 nm along them. Copyright 2000 Academic Press.

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Year:  2000        PMID： 11049752     DOI： 10.1006/prep.2000.1307

Source DB:  PubMed          Journal:  Protein Expr Purif        ISSN： 1046-5928            Impact factor:   1.650


  21 in total

1.  Proton detection for signal enhancement in solid-state NMR experiments on mobile species in membrane proteins.

Authors:  Meaghan E Ward; Emily Ritz; Mumdooh A M Ahmed; Vladimir V Bamm; George Harauz; Leonid S Brown; Vladimir Ladizhansky
Journal:  J Biomol NMR       Date:  2015-10-22       Impact factor: 2.835

2.  Cytoplasmic domain of human myelin protein zero likely folded as beta-structure in compact myelin.

Authors:  Xiaoyang Luo; Deepak Sharma; Hideyo Inouye; Daniel Lee; Robin L Avila; Mario Salmona; Daniel A Kirschner
Journal:  Biophys J       Date:  2006-12-01       Impact factor: 4.033

3.  Differences in susceptibility of MBP charge isomers to digestion by stromelysin-1 (MMP-3) and release of an immunodominant epitope.

Authors:  Cheryl A D'Souza; Mario A Moscarello
Journal:  Neurochem Res       Date:  2006-07-27       Impact factor: 3.996

4.  Structured functional domains of myelin basic protein: cross talk between actin polymerization and Ca(2+)-dependent calmodulin interaction.

Authors:  Vladimir V Bamm; Miguel De Avila; Graham S T Smith; Mumdooh A M Ahmed; George Harauz
Journal:  Biophys J       Date:  2011-09-07       Impact factor: 4.033

5.  Proline substitutions and threonine pseudophosphorylation of the SH3 ligand of 18.5-kDa myelin basic protein decrease its affinity for the Fyn-SH3 domain and alter process development and protein localization in oligodendrocytes.

Authors:  Graham S T Smith; Miguel De Avila; Pablo M Paez; Vilma Spreuer; Melanie K B Wills; Nina Jones; Joan M Boggs; George Harauz
Journal:  J Neurosci Res       Date:  2011-09-01       Impact factor: 4.164

6.  Interactions of the 18.5 kDa isoform of myelin basic protein with Ca2+-calmodulin: in vitro studies using gel shift assays.

Authors:  David S Libich; George Harauz
Journal:  Mol Cell Biochem       Date:  2002-12       Impact factor: 3.396

7.  Classical 18.5-and 21.5-kDa isoforms of myelin basic protein inhibit calcium influx into oligodendroglial cells, in contrast to golli isoforms.

Authors:  Graham S T Smith; Pablo M Paez; Vilma Spreuer; Celia W Campagnoni; Joan M Boggs; Anthony T Campagnoni; George Harauz
Journal:  J Neurosci Res       Date:  2011-01-13       Impact factor: 4.164

8.  Adduction of cholesterol 5,6-secosterol aldehyde to membrane-bound myelin basic protein exposes an immunodominant epitope.

Authors:  Natalie K Cygan; Johanna C Scheinost; Terry D Butters; Paul Wentworth
Journal:  Biochemistry       Date:  2011-02-28       Impact factor: 3.162

9.  Classic 18.5- and 21.5-kDa myelin basic protein isoforms associate with cytoskeletal and SH3-domain proteins in the immortalized N19-oligodendroglial cell line stimulated by phorbol ester and IGF-1.

Authors:  Graham S T Smith; Lopamudra Homchaudhuri; Joan M Boggs; George Harauz
Journal:  Neurochem Res       Date:  2012-01-17       Impact factor: 3.996

10.  Deimination of membrane-bound myelin basic protein in multiple sclerosis exposes an immunodominant epitope.

Authors:  Abdiwahab A Musse; Joan M Boggs; George Harauz
Journal:  Proc Natl Acad Sci U S A       Date:  2006-03-09       Impact factor: 11.205
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