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Literature DB >> 11044135

ATP binding and ATPase activities associated with recombinant rabbit hemorrhagic disease virus 2C-like polypeptide.

M S Marín¹, R Casais, J M Alonso, F Parra.

Abstract

The carboxy-terminal region of the rabbit hemorrhagic disease virus p37 polyprotein cleavage product has been expressed in Escherichia coli as a glutathione S-transferase (GST) fusion protein. The recombinant GST-Delta2C protein showed in vitro ATP-binding and ATPase activities. Site-directed mutagenesis studies of the conserved residues G(522) and T(529) in motif A, D(566) and E(567) in motif B, and K(600) in motif C were also performed. These results provide the first experimental characterization of a 2C-like ATPase activity in a member of the Caliciviridae.

Entities: Chemical Species

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Year: 2000 PMID： 11044135 PMCID： PMC110965 DOI： 10.1128/jvi.74.22.10846-10851.2000

Source DB: PubMed Journal: J Virol ISSN： 0022-538X Impact factor: 5.103

31 in total

1. The carboxy-terminal part of the NS 3 protein of the West Nile flavivirus can be isolated as a soluble protein after proteolytic cleavage and represents an RNA-stimulated NTPase.

Authors: G Wengler; G Wengler
Journal: Virology Date: 1991-10 Impact factor: 3.616

2. Structure of the recA protein-ADP complex.

Authors: R M Story; T A Steitz
Journal: Nature Date: 1992-01-23 Impact factor: 49.962

3. Identification and characterization of the virus causing rabbit hemorrhagic disease.

Authors: V F Ohlinger; B Haas; G Meyers; F Weiland; H J Thiel
Journal: J Virol Date: 1990-07 Impact factor: 5.103

4. Rabbit hemorrhagic disease virus--molecular cloning and nucleotide sequencing of a calicivirus genome.

Authors: G Meyers; C Wirblich; H J Thiel
Journal: Virology Date: 1991-10 Impact factor: 3.616

Review 5. Clinical and pathological features of viral haemorrhagic disease of rabbits and the European brown hare syndrome.

Authors: P S Marcato; C Benazzi; G Vecchi; M Galeotti; L Della Salda; G Sarli; P Lucidi
Journal: Rev Sci Tech Date: 1991-06 Impact factor: 1.181

6. Genetic analysis of an NTP-binding motif in poliovirus polypeptide 2C.

Authors: C Mirzayan; E Wimmer
Journal: Virology Date: 1992-08 Impact factor: 3.616

7. Analysis of the functional significance of amino acid residues in the putative NTP-binding pattern of the poliovirus 2C protein.

Authors: N L Teterina; K M Kean; A E Gorbalenya; V I Agol; M Girard
Journal: J Gen Virol Date: 1992-08 Impact factor: 3.891

8. RNA-stimulated NTPase activity associated with yellow fever virus NS3 protein expressed in bacteria.

Authors: P Warrener; J K Tamura; M S Collett
Journal: J Virol Date: 1993-02 Impact factor: 5.103

9. Refined crystal structure of the triphosphate conformation of H-ras p21 at 1.35 A resolution: implications for the mechanism of GTP hydrolysis.

Authors: E F Pai; U Krengel; G A Petsko; R S Goody; W Kabsch; A Wittinghofer
Journal: EMBO J Date: 1990-08 Impact factor: 11.598

10. Processing map and essential cleavage sites of the nonstructural polyprotein encoded by ORF1 of the feline calicivirus genome.

Authors: Stanislav V Sosnovtsev; Mark Garfield; Kim Y Green
Journal: J Virol Date: 2002-07 Impact factor: 5.103

ATP binding and ATPase activities associated with recombinant rabbit hemorrhagic disease virus 2C-like polypeptide.

1. The carboxy-terminal part of the NS 3 protein of the West Nile flavivirus can be isolated as a soluble protein after proteolytic cleavage and represents an RNA-stimulated NTPase.

2. Structure of the recA protein-ADP complex.

3. Identification and characterization of the virus causing rabbit hemorrhagic disease.

4. Rabbit hemorrhagic disease virus--molecular cloning and nucleotide sequencing of a calicivirus genome.

Review 5. Clinical and pathological features of viral haemorrhagic disease of rabbits and the European brown hare syndrome.

6. Genetic analysis of an NTP-binding motif in poliovirus polypeptide 2C.

7. Analysis of the functional significance of amino acid residues in the putative NTP-binding pattern of the poliovirus 2C protein.

8. RNA-stimulated NTPase activity associated with yellow fever virus NS3 protein expressed in bacteria.

9. Refined crystal structure of the triphosphate conformation of H-ras p21 at 1.35 A resolution: implications for the mechanism of GTP hydrolysis.

10. Mutational analysis of a DEAD box RNA helicase: the mammalian translation initiation factor eIF-4A.

1. Snow Mountain virus genome sequence and virus-like particle assembly.

2. ATP hydrolysis and AMP kinase activities of nonstructural protein 2C of human parechovirus 1.

3. Poliovirus 2C protein forms homo-oligomeric structures required for ATPase activity.

Review 4. Comprehensive review of human sapoviruses.

5. Site-directed mutants of human RECQ1 reveal functional importance of the zinc binding domain.

6. Nucleotide triphosphatase and RNA chaperone activities of murine norovirus NS3.

7. The genome of hawaii virus and its relationship with other members of the caliciviridae.

8. Characterization of an enteropathogenic bovine calicivirus representing a potentially new calicivirus genus.

9. A nucleotide binding motif in hepatitis C virus (HCV) NS4B mediates HCV RNA replication.

10. Processing map and essential cleavage sites of the nonstructural polyprotein encoded by ORF1 of the feline calicivirus genome.