Literature DB >> 15452248

A nucleotide binding motif in hepatitis C virus (HCV) NS4B mediates HCV RNA replication.

Shirit Einav1, Menashe Elazar, Tsafi Danieli, Jeffrey S Glenn.   

Abstract

Hepatitis C virus (HCV) is a major cause of viral hepatitis. There is no effective therapy for most patients. We have identified a nucleotide binding motif (NBM) in one of the virus's nonstructural proteins, NS4B. This structural motif binds and hydrolyzes GTP and is conserved across HCV isolates. Genetically disrupting the NBM impairs GTP binding and hydrolysis and dramatically inhibits HCV RNA replication. These results have exciting implications for the HCV life cycle and novel antiviral strategies.

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Year:  2004        PMID： 15452248      PMCID： PMC521822          DOI： 10.1128/JVI.78.20.11288-11295.2004

Source DB:  PubMed          Journal:  J Virol        ISSN： 0022-538X            Impact factor:   5.103


  32 in total

1.  Molecular and ultrastructural analysis of heavy membrane fractions associated with the replication of Kunjin virus RNA.

Authors:  P W Chu; E G Westaway
Journal:  Arch Virol       Date:  1992       Impact factor: 2.574

Review 2.  The ras protein family: evolutionary tree and role of conserved amino acids.

Authors:  A Valencia; P Chardin; A Wittinghofer; C Sander
Journal:  Biochemistry       Date:  1991-05-14       Impact factor: 3.162

3.  A new superfamily of putative NTP-binding domains encoded by genomes of small DNA and RNA viruses.

Authors:  A E Gorbalenya; E V Koonin; Y I Wolf
Journal:  FEBS Lett       Date:  1990-03-12       Impact factor: 4.124

Review 4.  Viral proteins containing the purine NTP-binding sequence pattern.

Authors:  A E Gorbalenya; E V Koonin
Journal:  Nucleic Acids Res       Date:  1989-11-11       Impact factor: 16.971

5.  Association of foot-and-mouth disease virus replicase with RNA template and cytoplasmic membranes.

Authors:  L H Lazarus; R Barzilai
Journal:  J Gen Virol       Date:  1974-05       Impact factor: 3.891

6.  Biological properties of human c-Ha-ras1 genes mutated at codon 12.

Authors:  P H Seeburg; W W Colby; D J Capon; D V Goeddel; A D Levinson
Journal:  Nature       Date:  1984 Nov 1-7       Impact factor: 49.962

7.  Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.

Authors:  D B Smith; K S Johnson
Journal:  Gene       Date:  1988-07-15       Impact factor: 3.688

8.  Genetic analysis of an NTP-binding motif in poliovirus polypeptide 2C.

Authors:  C Mirzayan; E Wimmer
Journal:  Virology       Date:  1992-08       Impact factor: 3.616

9.  Amphipathic helix-dependent localization of NS5A mediates hepatitis C virus RNA replication.

Authors:  Menashe Elazar; Kwang Ho Cheong; Ping Liu; Harry B Greenberg; Charles M Rice; Jeffrey S Glenn
Journal:  J Virol       Date:  2003-05       Impact factor: 5.103

10.  Alphavirus RNA replicase is located on the cytoplasmic surface of endosomes and lysosomes.

Authors:  S Froshauer; J Kartenbeck; A Helenius
Journal:  J Cell Biol       Date:  1988-12       Impact factor: 10.539
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  47 in total

1.  Dimerization of the hepatitis C virus nonstructural protein 4B depends on the integrity of an aminoterminal basic leucine zipper.

Authors:  Martin-Walter Welker; Christoph Welsch; Aline Meyer; Iris Antes; Mario Albrecht; Nicole Forestier; Bernd Kronenberger; Thomas Lengauer; Albrecht Piiper; Stefan Zeuzem; Christoph Sarrazin
Journal:  Protein Sci       Date:  2010-07       Impact factor: 6.725

Review 2.  Studying hepatitis C virus: making the best of a bad virus.

Authors:  Timothy L Tellinghuisen; Matthew J Evans; Thomas von Hahn; Shihyun You; Charles M Rice
Journal:  J Virol       Date:  2007-05-23       Impact factor: 5.103

3.  Palmitoylation and polymerization of hepatitis C virus NS4B protein.

Authors:  Guann-Yi Yu; Ki-Jeong Lee; Lu Gao; Michael M C Lai
Journal:  J Virol       Date:  2006-06       Impact factor: 5.103

4.  The hepatitis C virus (HCV) NS4B RNA binding inhibitor clemizole is highly synergistic with HCV protease inhibitors.

Authors:  Shirit Einav; Hadas D Sobol; Elizabeth Gehrig; Jeffrey S Glenn
Journal:  J Infect Dis       Date:  2010-07-01       Impact factor: 5.226

5.  Encoded library technology screening of hepatitis C virus NS4B yields a small-molecule compound series with in vitro replicon activity.

Authors:  Christopher Arico-Muendel; Zhengrong Zhu; Hamilton Dickson; Derek Parks; Jesse Keicher; Jianghe Deng; Leah Aquilani; Frank Coppo; Todd Graybill; Kenneth Lind; Andrew Peat; Michael Thomson
Journal:  Antimicrob Agents Chemother       Date:  2015-03-30       Impact factor: 5.191

6.  The hepatitis C virus NS4B protein can trans-complement viral RNA replication and modulates production of infectious virus.

Authors:  Daniel M Jones; Arvind H Patel; Paul Targett-Adams; John McLauchlan
Journal:  J Virol       Date:  2008-12-10       Impact factor: 5.103

7.  Preclinical Characterization and In Vivo Efficacy of GSK8853, a Small-Molecule Inhibitor of the Hepatitis C Virus NS4B Protein.

Authors:  Jeffrey J Pouliot; Michael Thomson; Mi Xie; Joseph Horton; John Johnson; David Krull; Amanda Mathis; Yoshio Morikawa; Derek Parks; Richard Peterson; Takashi Shimada; Elizabeth Thomas; Jessica Vamathevan; Stephanie Van Horn; Zhiping Xiong; Robert Hamatake; Andrew J Peat
Journal:  Antimicrob Agents Chemother       Date:  2015-08-10       Impact factor: 5.191

8.  Identification of a novel determinant for membrane association in hepatitis C virus nonstructural protein 4B.

Authors:  Jérôme Gouttenoire; Valérie Castet; Roland Montserret; Naveen Arora; Vincent Raussens; Jean-Marie Ruysschaert; Eric Diesis; Hubert E Blum; François Penin; Darius Moradpour
Journal:  J Virol       Date:  2009-04-08       Impact factor: 5.103

9.  Hepatitis C virus NS4B targets lipid droplets through hydrophobic residues in the amphipathic helices.

Authors:  Torahiko Tanaka; Kazumichi Kuroda; Masanori Ikeda; Takaji Wakita; Nobuyuki Kato; Makoto Makishima
Journal:  J Lipid Res       Date:  2013-01-12       Impact factor: 5.922

10.  An amphipathic alpha-helix at the C terminus of hepatitis C virus nonstructural protein 4B mediates membrane association.

Authors:  Jérôme Gouttenoire; Roland Montserret; Audrey Kennel; François Penin; Darius Moradpour
Journal:  J Virol       Date:  2009-08-19       Impact factor: 5.103
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