Literature DB >> 11030343

Structure and function of Cdc6/Cdc18: implications for origin recognition and checkpoint control.

J Liu1, C L Smith, D DeRyckere, K DeAngelis, G S Martin, J M Berger.   

Abstract

Cdc6/Cdc18 is a conserved and essential component of prereplication complexes. The 2.0 A crystal structure of an archaeal Cdc6 ortholog, in conjunction with a mutational analysis of the homologous Cdc18 protein from Schizosaccharomyces pombe, reveals novel aspects of Cdc6/Cdc18 function. Two domains of Cdc6 form an AAA+-type nucleotide binding fold that is observed bound to Mg.ADP. A third domain adopts a winged-helix fold similar to known DNA binding modules. Sequence comparisons show that the winged-helix domain is conserved in Orc1, and mutagenesis data demonstrate that this region of Cdc6/Cdc18 is required for function in vivo. Additional mutational analyses suggest that nucleotide binding and/or hydrolysis by Cdc6/Cdc18 is required not only for progression through S phase, but also for maintenance of checkpoint control during S phase.

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Year:  2000        PMID： 11030343     DOI： 10.1016/s1097-2765(00)00062-9

Source DB:  PubMed          Journal:  Mol Cell        ISSN： 1097-2765            Impact factor:   17.970


  80 in total

1.  Analysis of Cdc6 function in the assembly of mammalian prereplication complexes.

Authors:  Jeanette Gowen Cook; Chi-Hyun Park; Thomas W Burke; Gustavo Leone; James DeGregori; Amber Engel; Joseph R Nevins
Journal:  Proc Natl Acad Sci U S A       Date:  2002-01-22       Impact factor: 11.205

2.  Xenopus Cdc6 performs separate functions in initiating DNA replication.

Authors:  Natalya S Frolova; Nancy Schek; Nadia Tikhmyanova; Thomas R Coleman
Journal:  Mol Biol Cell       Date:  2002-04       Impact factor: 4.138

3.  Functional analysis of mutant and wild-type Drosophila origin recognition complex.

Authors:  I Chesnokov; D Remus; M Botchan
Journal:  Proc Natl Acad Sci U S A       Date:  2001-10-02       Impact factor: 11.205

4.  Structural basis of replication origin recognition by the DnaA protein.

Authors:  Norie Fujikawa; Hitoshi Kurumizaka; Osamu Nureki; Takaho Terada; Mikako Shirouzu; Tsutomu Katayama; Shigeyuki Yokoyama
Journal:  Nucleic Acids Res       Date:  2003-04-15       Impact factor: 16.971

5.  The structure of bacterial DnaA: implications for general mechanisms underlying DNA replication initiation.

Authors:  Jan P Erzberger; Michelle M Pirruccello; James M Berger
Journal:  EMBO J       Date:  2002-09-16       Impact factor: 11.598

6.  Biochemical characterization of Cdc6/Orc1 binding to the replication origin of the euryarchaeon Methanothermobacter thermoautotrophicus.

Authors:  Stephanie A Capaldi; James M Berger
Journal:  Nucleic Acids Res       Date:  2004-09-09       Impact factor: 16.971

7.  The NAD(+)-dependent Sir2p histone deacetylase is a negative regulator of chromosomal DNA replication.

Authors:  Donald L Pappas; Ryan Frisch; Michael Weinreich
Journal:  Genes Dev       Date:  2004-04-01       Impact factor: 11.361

8.  Identification of DNA-binding proteins using structural, electrostatic and evolutionary features.

Authors:  Guy Nimrod; András Szilágyi; Christina Leslie; Nir Ben-Tal
Journal:  J Mol Biol       Date:  2009-02-20       Impact factor: 5.469

9.  Identification and autonomous replication capability of a chromosomal replication origin from the archaeon Sulfolobus solfataricus.

Authors:  Patrizia Contursi; Francesca M Pisani; Andrei Grigoriev; Raffaele Cannio; Simonetta Bartolucci; Mosè Rossi
Journal:  Extremophiles       Date:  2004-08-05       Impact factor: 2.395

10.  Crystal structure of a near-full-length archaeal MCM: functional insights for an AAA+ hexameric helicase.

Authors:  Aaron S Brewster; Ganggang Wang; Xian Yu; William B Greenleaf; José María Carazo; Matthew Tjajadi; Michael G Klein; Xiaojiang S Chen
Journal:  Proc Natl Acad Sci U S A       Date:  2008-12-10       Impact factor: 11.205
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