| Literature DB >> 11030338 |
A V Mazin1, C J Bornarth, J A Solinger, W D Heyer, S C Kowalczykowski.
Abstract
Rad51 and Rad54 proteins are important for the repair of double-stranded DNA (dsDNA) breaks by homologous recombination in eukaryotes. Rad51 assembles on single-stranded DNA (ssDNA) to form a helical nucleoprotein filament that performs homologous pairing with dsDNA; Rad54 stimulates this pairing substantially. Here, we demonstrate that Rad54 acts in concert with the mature Rad51-ssDNA filament. Enhancement of DNA pairing by Rad54 is greatest at an equimolar ratio relative to Rad51 within the filament. Reciprocally, the Rad51-ssDNA filament enhances both the dsDNA-dependent ATPase and the dsDNA unwinding activities of Rad54. We conclude that Rad54 participates in the DNA homology search as a component of the Rad51-nucleoprotein filament and that the filament delivers Rad54 to the dsDNA pairing locus, thereby linking the unwinding of potential target DNA with the homology search process.Entities:
Mesh：
Substances：
Year: 2000 PMID： 11030338 DOI： 10.1016/s1097-2765(00)00057-5
Source DB: PubMed Journal: Mol Cell ISSN： 1097-2765 Impact factor: 17.970