| Literature DB >> 11024192 |
D V Rebrikov1, O V Britanova, N G Gurskaya, K A Lukyanov, V S Tarabykin, S A Lukyanov.
Abstract
Suppression subtractive hybridization (SSH) is one of the most powerful and popular methods for isolating differentially expressed transcripts. However, SSH-generated libraries typically contain some background clones representing non-differentially expressed transcripts. To overcome this problem we developed a simple procedure that substantially decreases the number of background clones. This method is based on the following difference between target and background cDNAs: each kind of background molecule has only one orientation with respect to the two different flanking adapter sequences used in SSH, while truly differentially expressed target cDNA fragments are represented by both sequence orientations. The described method selects the molecules that arose due to hybridization of such mirror-orientated molecules. The efficiency of this method was demonstrated in both model and real experimental subtractions.Mesh：
Substances：
Year: 2000 PMID： 11024192 PMCID： PMC110806 DOI： 10.1093/nar/28.20.e90
Source DB: PubMed Journal: Nucleic Acids Res ISSN： 0305-1048 Impact factor: 16.971