Literature DB >> 11024187

Amplification of IgG VH and VL (Fab) from single human plasma cells and B cells.

J A Coronella1, P Telleman, T D Truong, F Ylera, R P Junghans.   

Abstract

Amplification of human immunoglobulin has many potential applications such as analysis of clonality, isolation of immunogenic antigens and antigen-specific immunotherapy. Here we describe a method for amplification of human immunoglobulin heavy and light chains from single B lymphocytes or plasma cells. Cells are isolated by FACS, and Ig is amplified by semi-nested RT-PCR. The method is versatile, sensitive and reliable: it provides appropriately paired heavy and light chains, requiring as little as 2 days to produce amplified Fab DNA from human tissues.

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Year:  2000        PMID： 11024187      PMCID： PMC110801          DOI： 10.1093/nar/28.20.e85

Source DB:  PubMed          Journal:  Nucleic Acids Res        ISSN： 0305-1048            Impact factor:   16.971


  13 in total

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2.  Sequencing heavy- and light-chain variable genes of single B-hybridoma cells by total enzymatic amplification.

Authors:  A H Liu; G Creadon; L J Wysocki
Journal:  Proc Natl Acad Sci U S A       Date:  1992-08-15       Impact factor: 11.205

3.  Antibody redesign by chain shuffling from random combinatorial immunoglobulin libraries.

Authors:  A S Kang; T M Jones; D R Burton
Journal:  Proc Natl Acad Sci U S A       Date:  1991-12-15       Impact factor: 11.205

4.  A simplified method for single-cell RT-PCR that can detect and distinguish genomic DNA and mRNA transcripts.

Authors:  J Tong; S Bendahhou; H Chen; W S Agnew
Journal:  Nucleic Acids Res       Date:  1994-08-11       Impact factor: 16.971

5.  Single, antigen-specific B cells used to generate Fab fragments using CD40-mediated amplification or direct PCR cloning.

Authors:  A C Lagerkvist; C Furebring; C A Borrebaeck
Journal:  Biotechniques       Date:  1995-05       Impact factor: 1.993

6.  The use of reverse transcription polymerase chain reaction to analyse large numbers of mRNA species from a single cell.

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Review 7.  Phage libraries--a new route to clinically useful antibodies.

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8.  Development of the antibody repertoire as revealed by single-cell PCR of FACS-sorted B-cell subsets.

Authors:  A B Kantor; C E Merrill; J D MacKenzie; L A Herzenberg; J L Hillson
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Review 9.  Human antibodies from combinatorial libraries.

Authors:  D R Burton; C F Barbas
Journal:  Adv Immunol       Date:  1994       Impact factor: 3.543

10.  A novel strategy for generating monoclonal antibodies from single, isolated lymphocytes producing antibodies of defined specificities.

Authors:  J S Babcook; K B Leslie; O A Olsen; R A Salmon; J W Schrader
Journal:  Proc Natl Acad Sci U S A       Date:  1996-07-23       Impact factor: 11.205
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Review 2.  Accessing the human repertoire for broadly neutralizing HIV antibodies.

Authors:  Philip W Hammond
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4.  The rapid generation of recombinant functional monoclonal antibodies from individual, antigen-specific bone marrow-derived plasma cells isolated using a novel fluorescence-based method.

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6.  Prognostic B-cell signatures using mRNA-seq in patients with subtype-specific breast and ovarian cancer.

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7.  B cells, antibody-secreting cells, and virus-specific antibodies respond to herpes simplex virus 2 reactivation in skin.

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8.  Rapid production of antigen-specific monoclonal antibodies from a variety of animals.

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9.  Target-selective homologous recombination cloning for high-throughput generation of monoclonal antibodies from single plasma cells.

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Review 10.  Development of Therapeutic Antibodies and Modulating the Characteristics of Therapeutic Antibodies to Maximize the Therapeutic Efficacy.

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