| Literature DB >> 10990024 |
M Albert1, W Repetschnigg, J Ortner, J Gomes, B J Paul, C Illaszewicz, H Weber, W Steiner, K Dax.
Abstract
A fast method for the simultaneous detection of different glycosidolytic activities in commercially available enzyme preparations and crude culture filtrates was found in using, as substrate, a mixture of different glycosyl fluorides and 19F NMR spectroscopy as a screening technique. Accompanying studies regarding the hydrolytic stability of these fluorides in various buffer systems, as well as conditions of their long-term storage, were carried out. A simple procedure for the preparation of beta-D-mannopyranosyl fluoride in gram quantities is given.Entities:
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Year: 2000 PMID： 10990024 DOI： 10.1016/s0008-6215(00)00060-4
Source DB: PubMed Journal: Carbohydr Res ISSN： 0008-6215 Impact factor: 2.104