OBJECTIVE: To determine the contribution of urethral and skin flora to seminal fluid cultures and the relation between bacteriospermia and seminal leukocytes. DESIGN: Prospective study. SETTING: IVF-ET unit at a university teaching hospital. PATIENT(S): Sixty men starting an IVF-ET program. INTERVENTION(S): Culture of sequential first-catch urine, midstream urine, and semen samples with evaluation of seminal leukocytes. MAIN OUTCOME MEASURE(S): A comparison of microbes from first-catch urine, midstream urine, and semen samples and the correlations of seminal microbes, elevated leukocyte concentrations, and pregnancy. RESULT(S): Microorganisms were detected in 37% of first-catch urine samples, 27% of midstream urine samples, and 51% of semen samples. Most microorganisms were gram-positive microbes and were common to both urine and semen samples. Mean and median leukocyte concentrations were 0.98 x 10(6)/mL and 0.10 x 10(6)/mL, respectively. There was no correlation between seminal microbes and raised leukocytes or between leukocytospermia and/or bacteriospermia and pregnancy. CONCLUSION(S): Microorganisms are commonly found in insignificant quantities in the semen of asymptomatic men. The frequent isolation of gram-positive microbes common to both urine and semen and the absence of a correlation with raised leukocyte concentrations suggest that bacteriospermia most commonly represents contamination.
OBJECTIVE: To determine the contribution of urethral and skin flora to seminal fluid cultures and the relation between bacteriospermia and seminal leukocytes. DESIGN: Prospective study. SETTING: IVF-ET unit at a university teaching hospital. PATIENT(S): Sixty men starting an IVF-ET program. INTERVENTION(S): Culture of sequential first-catch urine, midstream urine, and semen samples with evaluation of seminal leukocytes. MAIN OUTCOME MEASURE(S): A comparison of microbes from first-catch urine, midstream urine, and semen samples and the correlations of seminal microbes, elevated leukocyte concentrations, and pregnancy. RESULT(S): Microorganisms were detected in 37% of first-catch urine samples, 27% of midstream urine samples, and 51% of semen samples. Most microorganisms were gram-positive microbes and were common to both urine and semen samples. Mean and median leukocyte concentrations were 0.98 x 10(6)/mL and 0.10 x 10(6)/mL, respectively. There was no correlation between seminal microbes and raised leukocytes or between leukocytospermia and/or bacteriospermia and pregnancy. CONCLUSION(S): Microorganisms are commonly found in insignificant quantities in the semen of asymptomatic men. The frequent isolation of gram-positive microbes common to both urine and semen and the absence of a correlation with raised leukocyte concentrations suggest that bacteriospermia most commonly represents contamination.
Authors: G Luke Machen; Erin T Bird; Monica L Brown; Dale A Ingalsbe; Milaida M East; Michelle Reyes; Thomas J Kuehl Journal: Proc (Bayl Univ Med Cent) Date: 2018-03-20
Authors: Stuart Wigby; Susan S Suarez; Brian P Lazzaro; Tommaso Pizzari; Mariana F Wolfner Journal: Curr Top Dev Biol Date: 2019-05-15 Impact factor: 4.897
Authors: A Rusz; A Pilatz; F Wagenlehner; T Linn; Th Diemer; H C Schuppe; J Lohmeyer; H Hossain; W Weidner Journal: World J Urol Date: 2011-07-12 Impact factor: 4.226
Authors: Guntram Bezold; Joseph A Politch; Nancy B Kiviat; Jane M Kuypers; Hans Wolff; Deborah J Anderson Journal: Fertil Steril Date: 2007-04-11 Impact factor: 7.329
Authors: Oleg V Bukharin; Natalya B Perunova; Elena V Ivanova; Irina N Chaynikova; Anastasia V Bekpergenova; Taisiya A Bondarenko; Michael D Kuzmin Journal: Asian J Androl Date: 2022 Jul-Aug Impact factor: 3.054