B Vasarhelyi1, T Tulassay, A Ver, M Dobos, I Kocsis, I Seri. 1. Joint Research Program of the Hungarian Academy of Sciences and the 1st Department of Pediatrics, Semmelweis University School of Medicine, Budapest, Hungary.
Abstract
AIM: To study the relation between erythrocyte Na(+),K(+)-ATPase subunit isoform composition, Na(+),K(+)-ATPase activity, and cation pump function in preterm and term neonates. DESIGN: Erythrocyte Na(+),K(+)-ATPase subunit isoform abundance, Na(+),K(+)-ATPase activity, and cation pump function were studied in blood samples obtained from 56 preterm neonates of 28-32 weeks gestation (group 1), 58 preterm neonates of 33-36 weeks gestation (group 2), and 122 term neonates (group 3) during the first two postnatal days. RESULTS: alpha(1) isoform abundance was higher and beta(2) isoform abundance was lower in group 1 than in group 3 (p = 0.0002). alpha(2) and beta(1) isoform abundance did not change with maturation and there was no evidence for the presence of the alpha(3) isoform. Gestational age was inversely related to Na(+), K(+)-ATPase activity (p = 0.0001) and directly related to intracellular Na(+) concentration (p = 0.0025). CONCLUSIONS: Expression of the alpha(1) and beta(2) Na(+),K(+)-ATPase subunit isoforms is developmentally regulated. The increased abundance of alpha(1) isoforms of immature neonates translates to increased ATPase activity. The lower intracellular Na(+) concentration of immature neonates suggests that their erythrocyte Na(+),K(+)-ATPase cation pump function may also be increased.
AIM: To study the relation between erythrocyte Na(+),K(+)-ATPase subunit isoform composition, Na(+),K(+)-ATPase activity, and cation pump function in preterm and term neonates. DESIGN: Erythrocyte Na(+),K(+)-ATPase subunit isoform abundance, Na(+),K(+)-ATPase activity, and cation pump function were studied in blood samples obtained from 56 preterm neonates of 28-32 weeks gestation (group 1), 58 preterm neonates of 33-36 weeks gestation (group 2), and 122 term neonates (group 3) during the first two postnatal days. RESULTS: alpha(1) isoform abundance was higher and beta(2) isoform abundance was lower in group 1 than in group 3 (p = 0.0002). alpha(2) and beta(1) isoform abundance did not change with maturation and there was no evidence for the presence of the alpha(3) isoform. Gestational age was inversely related to Na(+), K(+)-ATPase activity (p = 0.0001) and directly related to intracellular Na(+) concentration (p = 0.0025). CONCLUSIONS: Expression of the alpha(1) and beta(2) Na(+),K(+)-ATPase subunit isoforms is developmentally regulated. The increased abundance of alpha(1) isoforms of immature neonates translates to increased ATPase activity. The lower intracellular Na(+) concentration of immature neonates suggests that their erythrocyte Na(+),K(+)-ATPase cation pump function may also be increased.
Authors: T Bistritzer; M Berkovitch; M J Rappoport; S Evans; S Arieli; M Goldberg; I Tavori; M Aladjem Journal: Arch Dis Child Fetal Neonatal Ed Date: 1999-11 Impact factor: 5.747
Authors: J L Stefano; M E Norman; M C Morales; J M Goplerud; O P Mishra; M Delivoria-Papadopoulos Journal: J Pediatr Date: 1993-02 Impact factor: 4.406
Authors: Joseph F Hoffman; Amittha Wickrema; Olga Potapova; Mark Milanick; Douglas R Yingst Journal: Proc Natl Acad Sci U S A Date: 2002-10-18 Impact factor: 11.205
Authors: Vasilios I Giapros; Agathoklis A Tsatsoulis; Ekaterini A Drougia; Konstantinos D Kollios; Ekaterini C Siomou; Styliani K Andronikou Journal: Pediatr Nephrol Date: 2004-06-04 Impact factor: 3.714