The ischemic heart--experimental models.

Results obtained by experimental studies of the ischemic heart have been of tremendous importance for the understanding of physiology, biochemistry and lately also the molecular genetics of the heart. Experimental models in use for the study of the ischemic heart involve studies on the integrated organism, experiments with isolated hearts or multicellular preparation, and also studies of cells isolated from the heart. Regional ischemia in the anaesthetized animal has been a standard model. Knowledge about infarct size limitation as well as heart function in acute and chronic ischemia has been obtained based on experiments in a wide variety of species. The isolated perfused heart has been subjected to extensive use. As a result, the understanding of intracellular processes is constantly developing. Cell models and transgenic-mice models represent promising additions. Each model and each species has certain advantages and disadvantages. Variability in susceptibility towards ischemia and reperfusion is also present. The consequences of ischemia can be described as contractile dysfunction and stunning, arrhythmia and infarction each representing different endpoints of injury. The experimental model is also heavily dependent on the endpoint that is chosen for the study. Results obtained in one experimental model can, therefore, not be generalized into universal conclusions about the ischemic heart. With respect to the human and the disease caused by myocardial ischemia, fragments of knowledge put together from different types of experimental models create the background for successful design of potential treatment. Copyright 2000 Academic Press.