Crystallization and preliminary X-ray analysis of Clostridium botulinum neurotoxin type B.

Single crystals of Clostridium botulinum neurotoxin type B have been obtained by the vapor-diffusion method. These crystals belong to space group P2(1), with unit-cell parameters a = 76.08, b = 123.11, c = 95.86 A, beta = 113.03 degrees and diffract to at least 1.8 A resolution. Native data have been collected from flash-frozen crystals at the National Synchrotron facility of Brookhaven National Laboratory. These crystals often tend to be non-isomorphic.