OBJECTIVE: To assess whether genetic polymorphisms implicated as risk factors for other tobacco-associated malignancies are associated with altered risk of head and neck squamous cell carcinoma. DESIGN: Case-control study. SUBJECTS: One hundred sixty patients with head and neck squamous cell carcinoma recruited from a university-based head and neck oncology clinic and 149 population-based controls. METHODS: Genotyping of the CYP1A1 (Ile462Val), GSTM1 (null), GSTP1 (Ile105Val), GSTT1 (null), and P53 (Arg72Pro) genes was performed by polymerase chain reaction-based techniques on DNA prepared from peripheral blood. In addition, a questionnaire was used to collect demographic information from each subject. RESULTS: Cases were significantly older (p <.0001) and had significantly greater tobacco use (p <.0001) and were more likely to be male (p <.0001) than were control subjects, thus confirming known risk factors for this disease. When cases and controls were compared by simple chi-square analysis, only the frequency of CYP1A1 (Ile462Val) polymorphism was significantly different between cases and controls (OR =.42; 95% CI =.18-.99; p <.04). However, with a logistic regression model to control for known risk factors, we were unable to demonstrate a significant association with head and neck cancer for any of the polymorphisms tested, including CYP1A1. CONCLUSIONS: This population fails to identify a relationship between the above-mentioned polymorphisms and head and neck cancer.
OBJECTIVE: To assess whether genetic polymorphisms implicated as risk factors for other tobacco-associated malignancies are associated with altered risk of head and neck squamous cell carcinoma. DESIGN: Case-control study. SUBJECTS: One hundred sixty patients with head and neck squamous cell carcinoma recruited from a university-based head and neck oncology clinic and 149 population-based controls. METHODS: Genotyping of the CYP1A1 (Ile462Val), GSTM1 (null), GSTP1 (Ile105Val), GSTT1 (null), and P53 (Arg72Pro) genes was performed by polymerase chain reaction-based techniques on DNA prepared from peripheral blood. In addition, a questionnaire was used to collect demographic information from each subject. RESULTS: Cases were significantly older (p <.0001) and had significantly greater tobacco use (p <.0001) and were more likely to be male (p <.0001) than were control subjects, thus confirming known risk factors for this disease. When cases and controls were compared by simple chi-square analysis, only the frequency of CYP1A1 (Ile462Val) polymorphism was significantly different between cases and controls (OR =.42; 95% CI =.18-.99; p <.04). However, with a logistic regression model to control for known risk factors, we were unable to demonstrate a significant association with head and neck cancer for any of the polymorphisms tested, including CYP1A1. CONCLUSIONS: This population fails to identify a relationship between the above-mentioned polymorphisms and head and neck cancer.
Authors: G Cadoni; S Boccia; L Petrelli; P Di Giannantonio; D Arzani; A Giorgio; E De Feo; M Pandolfini; P Gallì; G Paludetti; G Ricciardi Journal: Acta Otorhinolaryngol Ital Date: 2012-02 Impact factor: 2.124
Authors: Leonor Varela-Lema; Emanuela Taioli; Alberto Ruano-Ravina; Juan M Barros-Dios; Devasena Anantharaman; Simone Benhamou; Stefania Boccia; Rajani A Bhisey; Gabriella Cadoni; Ettore Capoluongo; Chien-Jen Chen; William Foulkes; Eny Maria Goloni-Bertollo; Ana Hatagima; Richard B Hayes; Takahiko Katoh; Sergio Koifman; Phillip Lazarus; Johannes J Manni; Manoj Mahimkar; Shunji Morita; Jong Park; Kwang-Kyun Park; Erika Cristina Pavarino Bertelli; Enilze Maria de Souza Fonseca Ribeiro; Bidyut Roy; Margaret R Spitz; Richard C Strange; Qingyi Wei; Camille C Ragin Journal: Genet Med Date: 2008-06 Impact factor: 8.822