Literature DB >> 10939408

Rapid and direct sequencing of double-stranded DNA using exonuclease III and MALDI-TOF MS.

U Puapaiboon1, J Jai-nhuknan, J A Cowan.   

Abstract

Application of MALDI-TOF MS to direct sequencing of dsDNA substrates is demonstrated using a strategy that employs exonuclease III digestion of a target sequence. Experimental conditions for exonuclease III have been optimized for this application, including addition of essential divalent metal ion cofactors. A short cation-exchange column was designed to provide efficient sample cleanup and overcome major problems arising from salt interference.

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Year:  2000        PMID： 10939408     DOI： 10.1021/ac000181n

Source DB:  PubMed          Journal:  Anal Chem        ISSN： 0003-2700            Impact factor:   6.986


  3 in total

1.  Using surface-assisted laser desorption/ionization mass spectrometry to detect ss- and ds-oligodeoxynucleotides.

Authors:  Wen-Tsen Chen; Ming-Feng Huang; Huan-Tsung Chang
Journal:  J Am Soc Mass Spectrom       Date:  2013-03-29       Impact factor: 3.109

2.  Crystallization and preliminary X-ray characterization of two thermostable DNA nucleases.

Authors:  E Bartholomeus Kuettner; Sven Pfeifer; Antje Keim; Thomas Greiner-Stöffele; Norbert Sträter
Journal:  Acta Crystallogr Sect F Struct Biol Cryst Commun       Date:  2006-11-30

3.  Characterization of a multi-functional metal-mediated nuclease by MALDI-TOF mass spectrometry.

Authors:  U Puapaiboon; J Jai-Nhuknan; J A Cowan
Journal:  Nucleic Acids Res       Date:  2001-09-01       Impact factor: 16.971
  3 in total

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