Literature DB >> 10932175

Hot-stop PCR: a simple and general assay for linear quantitation of allele ratios.

H Uejima1, M P Lee, H Cui, A P Feinberg.   

Abstract

We have developed a simple, quantitative assay for measurement of allele ratios that circumvents the problem of heteroduplex formation skewing the results of restriction endonuclease digestion of PCR products. This assay, 'hot-stop PCR', involves addition of a radiolabelled PCR primer at the final cycle. We applied the assay to analysis of loss of imprinting (LOI) of the insulin-like growth factor II gene (IGF2) in tumours.

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Year:  2000        PMID： 10932175     DOI： 10.1038/78040

Source DB:  PubMed          Journal:  Nat Genet        ISSN： 1061-4036            Impact factor:   38.330


  38 in total

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Journal:  Mamm Genome       Date:  2005-10-29       Impact factor: 2.957

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Authors:  Jong-Yeon Shin; Galina V Fitzpatrick; Michael J Higgins
Journal:  EMBO J       Date:  2007-12-13       Impact factor: 11.598

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9.  Identification of a large novel imprinted gene cluster on mouse proximal chromosome 6.

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10.  Determining the one, two, three, or four long and short loci of human complement C4 in a major histocompatibility complex haplotype encoding C4A or C4B proteins.

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Journal:  Am J Hum Genet       Date:  2002-09-10       Impact factor: 11.025
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