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Literature DB >> 10921985

Simple and reliable multiplex PCR assay for surveillance isolates of vancomycin-resistant enterococci.

R Kariyama¹, R Mitsuhata, J W Chow, D B Clewell, H Kumon.

Abstract

Conditions have been optimized for the use of a multiplex PCR for the detection of vancomycin-resistant enterococci in nosocomial surveillance specimens. Seven primer sets targeting the genes vanA, vanB, vanC1, vanC2/C3 Enterococcus faecalis-specific, Enterococcus faecium-specific, and rrs (16S rRNA) were used in one reaction tube. The PCR method developed in the present study is simple and reliable for the rapid characterization of vancomycin-resistant enterococci.

Entities: Chemical Gene Species

Mesh：

Substances：
RNA, Ribosomal, 16S

Year: 2000 PMID： 10921985 PMCID： PMC87194

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

12 in total

1. Detection of glycopeptide resistance genotypes and identification to the species level of clinically relevant enterococci by PCR.

Authors: S Dutka-Malen; S Evers; P Courvalin
Journal: J Clin Microbiol Date: 1995-01 Impact factor: 5.948

2. First report of the isolation of high-level vancomycin-resistant Enterococcus faecium from a patient in Japan.

Authors: N Fujita; M Yoshimura; T Komori; K Tanimoto; Y Ike
Journal: Antimicrob Agents Chemother Date: 1998-08 Impact factor: 5.191

3. An internal control for routine diagnostic PCR: design, properties, and effect on clinical performance.

Authors: M Rosenstraus; Z Wang; S Y Chang; D DeBonville; J P Spadoro
Journal: J Clin Microbiol Date: 1998-01 Impact factor: 5.948

4. Detection of vancomycin-resistant enterococci in fecal samples by PCR.

Authors: S Satake; N Clark; D Rimland; F S Nolte; F C Tenover
Journal: J Clin Microbiol Date: 1997-09 Impact factor: 5.948

5. In-vitro activity of arbekacin alone and in combination with vancomycin against gentamicin- and methicillin-resistant Staphylococcus aureus.

Authors: I You; R Kariyama; M J Zervos; H Kumon; J W Chow
Journal: Diagn Microbiol Infect Dis Date: 2000-01 Impact factor: 2.803

6. Rapid characterization schemes for surveillance isolates of vancomycin-resistant enterococci.

Authors: D F Sahm; L Free; C Smith; M Eveland; L M Mundy
Journal: J Clin Microbiol Date: 1997-08 Impact factor: 5.948

7. Evaluation of a vanA-specific PCR assay for detection of vancomycin-resistant Enterococcus faecium during a hospital outbreak.

Authors: M Roger; M C Faucher; P Forest; P St-Antoine; F Coutlée
Journal: J Clin Microbiol Date: 1999-10 Impact factor: 5.948

8. A PCR assay for identification of Enterococcus faecium.

Authors: S Cheng; F K McCleskey; M J Gress; J M Petroziello; R Liu; H Namdari; K Beninga; A Salmen; V G DelVecchio
Journal: J Clin Microbiol Date: 1997-05 Impact factor: 5.948

9. Molecular analysis of glycopeptide-resistant Enterococcus faecium isolates collected from Michigan hospitals over a 6-year period.

Authors: L Thal; S Donabedian; B Robinson-Dunn; J W Chow; L Dembry; D B Clewell; D Alshab; M J Zervos
Journal: J Clin Microbiol Date: 1998-11 Impact factor: 5.948

10. Characterization of glycopeptide-resistant enterococci from U.S. hospitals.

Authors: N C Clark; R C Cooksey; B C Hill; J M Swenson; F C Tenover
Journal: Antimicrob Agents Chemother Date: 1993-11 Impact factor: 5.191

82 in total

1. Routine molecular identification of enterococci by gene-specific PCR and 16S ribosomal DNA sequencing.

Authors: S Angeletti; G Lorino; G Gherardi; F Battistoni; M De Cesaris ; G Dicuonzo
Journal: J Clin Microbiol Date: 2001-02 Impact factor: 5.948

2. Identification of a Tn1546-like (type 2) element in vancomycin-resistant Enterococcus faecium isolated from hospitalized patients in Japan.

Authors: R Kariyama; H Kuman; A M Hammerum; F M Aarestrup; L B Jensen
Journal: Antimicrob Agents Chemother Date: 2001-03 Impact factor: 5.191

3. Improved primer design for multiplex PCR analysis of vancomycin-resistant Enterococcus spp.

Authors: S Elsayed; N Hamilton; D Boyd; M Mulvey
Journal: J Clin Microbiol Date: 2001-06 Impact factor: 5.948

4. Evaluation of 15 motility media and a direct microscopic method for detection of motility in enterococci.

Authors: Kenneth Van Horn; Clara Tóth; Reiko Kariyama; Ritsuko Mitsuhata; Hiromi Kumon
Journal: J Clin Microbiol Date: 2002-07 Impact factor: 5.948

5. Identification with multiplex PCR assay of enterococcus species isolated from dairy products in Umbria, Italy.

Authors: B T Cenci Goga; L Aquilanti; A Osimani; D Miraglia; F Aloisio
Journal: Vet Res Commun Date: 2003-09 Impact factor: 2.459

6. Relationship between phenotypic and genotypic florfenicol resistance in Escherichia coli.

Authors: Randall S Singer; Sheila K Patterson; Anne E Meier; Jessica K Gibson; Hannah L Lee; Carol W Maddox
Journal: Antimicrob Agents Chemother Date: 2004-10 Impact factor: 5.191

7. Ecology of antibiotic resistance genes: characterization of enterococci from houseflies collected in food settings.

Authors: Lilia Macovei; Ludek Zurek
Journal: Appl Environ Microbiol Date: 2006-06 Impact factor: 4.792

8. Evaluation of a new molecular system for simultaneous identification of four Enterococcus species and their glycopeptide resistance genotypes.

Authors: U Eigner; A Fahr; M Weizenegger; W Witte
Journal: J Clin Microbiol Date: 2005-06 Impact factor: 5.948

9. Utility of direct, real-time PCR in the management of a nosocomial outbreak of vancomycin-resistant Enterococcus faecium (vanB genotype).

Authors: H A Al-Mohri; M A Tadros; L Louie; M Vearncombe; A E Simor
Journal: Eur J Clin Microbiol Infect Dis Date: 2007-12-18 Impact factor: 3.267

10. Influx of enterococci and associated antibiotic resistance and virulence genes from ready-to-eat food to the human digestive tract.

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Journal: Appl Environ Microbiol Date: 2007-08-31 Impact factor: 4.792