Literature DB >> 10919829

PCR use of highly conserved DNA regions for identification of Sinorhizobium meliloti.

M Sánchez-Contreras1, J Lloret, M Martín, M Villacieros, I Bonilla, R Rivilla.   

Abstract

A PCR identification method in which four primers that recognize homologous conserved regions in the Sinorhizobium meliloti genome are used was developed and tested. The regions used for identification were the nodbox 4 locus, which is located in one of the symbiotic megaplasmids, and the mucR gene, which is located in the chromosome. The new method was used to establish a collection of S. meliloti strains from polluted soils.

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Year:  2000        PMID： 10919829      PMCID： PMC92193          DOI： 10.1128/AEM.66.8.3621-3623.2000

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  13 in total

1.  MucR is necessary for galactoglucan production in Sinorhizobium meliloti EFB1.

Authors:  M Martín; J Lloret; M Sánchez-Contreras; I Bonilla; R Rivilla
Journal:  Mol Plant Microbe Interact       Date:  2000-01       Impact factor: 4.171

2.  Effect of the concentration of sodium chloride in the medium on the relative proportions of poly- and oligo-saccharides excreted by Rhizobium meliloti strain YE-2SL.

Authors:  L P Zevenhuizen; P Faleschini
Journal:  Carbohydr Res       Date:  1991-01-15       Impact factor: 2.104

Review 3.  Regulation of symbiotic root nodule development.

Authors:  M Schultze; A Kondorosi
Journal:  Annu Rev Genet       Date:  1998       Impact factor: 16.830

4.  Evaluation of the resolving power of three different DNA fingerprinting methods to discriminate among isolates of a natural Rhizobium meliloti population.

Authors:  S Niemann; A Pühler; H V Tichy; R Simon; W Selbitschka
Journal:  J Appl Microbiol       Date:  1997-04       Impact factor: 3.772

5.  Genetic basis of enterobacterial repetitive intergenic consensus (ERIC)-PCR fingerprint pattern in Sinorhizobium meliloti and identification of S. meliloti employing PCR primers derived from an ERIC-PCR fragment.

Authors:  S Niemann; T Dammann-Kalinowski; A Nagel; A Pühler; W Selbitschka
Journal:  Arch Microbiol       Date:  1999-07       Impact factor: 2.552

6.  rosR, a determinant of nodulation competitiveness in Rhizobium etli.

Authors:  M A Bittinger; J L Milner; B J Saville; J Handelsman
Journal:  Mol Plant Microbe Interact       Date:  1997-03       Impact factor: 4.171

7.  Molecular basis of symbiosis between Rhizobium and legumes.

Authors:  C Freiberg; R Fellay; A Bairoch; W J Broughton; A Rosenthal; X Perret
Journal:  Nature       Date:  1997-05-22       Impact factor: 49.962

8.  Molecular analysis of the Rhizobium meliloti mucR gene regulating the biosynthesis of the exopolysaccharides succinoglycan and galactoglucan.

Authors:  M Keller; A Roxlau; W M Weng; M Schmidt; J Quandt; K Niehaus; D Jording; W Arnold; A Pühler
Journal:  Mol Plant Microbe Interact       Date:  1995 Mar-Apr       Impact factor: 4.171

9.  Ionic Stress and Osmotic Pressure Induce Different Alterations in the Lipopolysaccharide of a Rhizobium meliloti Strain.

Authors:  J Lloret; L Bolanos; M M Lucas; J M Peart; N J Brewin; I Bonilla; R Rivilla
Journal:  Appl Environ Microbiol       Date:  1995-10       Impact factor: 4.792

10.  Pleiotropic effects of regulatory ros mutants of Agrobacterium radiobacter and their interaction with Fe and glucose.

Authors:  G Brightwell; H Hussain; A Tiburtius; K H Yeoman; A W Johnston
Journal:  Mol Plant Microbe Interact       Date:  1995 Sep-Oct       Impact factor: 4.171
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