Literature DB >> 10892647

Structural organization of the RNA polymerase-promoter open complex.

N Naryshkin1, A Revyakin, Y Kim, V Mekler, R H Ebright.   

Abstract

We have used systematic site-specific protein-DNA photocrosslinking to define interactions between bacterial RNA polymerase (RNAP) and promoter DNA in the catalytically competent RNAP-promoter open complex (RPo). We have mapped more than 100 distinct crosslinks between individual segments of RNAP subunits and individual phosphates of promoter DNA. The results provide a comprehensive description of protein-DNA interactions in RPo, permit construction of a detailed model for the structure of RPo, and permit analysis of effects of a transcriptional activator on the structure of RPo.

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Year:  2000        PMID： 10892647     DOI： 10.1016/s0092-8674(00)80872-7

Source DB:  PubMed          Journal:  Cell        ISSN： 0092-8674            Impact factor:   41.582


  65 in total

1.  Fine structure of E. coli RNA polymerase-promoter interactions: alpha subunit binding to the UP element minor groove.

Authors:  W Ross; A Ernst; R L Gourse
Journal:  Genes Dev       Date:  2001-03-01       Impact factor: 11.361

2.  Mode of DNA-protein interaction between the C-terminal domain of Escherichia coli RNA polymerase alpha subunit and T7D promoter UP element.

Authors:  O N Ozoline; N Fujita; A Ishihama
Journal:  Nucleic Acids Res       Date:  2001-12-15       Impact factor: 16.971

3.  Structure-based analysis of RNA polymerase function: the largest subunit's rudder contributes critically to elongation complex stability and is not involved in the maintenance of RNA-DNA hybrid length.

Authors:  Konstantin Kuznedelov; Nataliya Korzheva; Arkady Mustaev; Konstantin Severinov
Journal:  EMBO J       Date:  2002-03-15       Impact factor: 11.598

4.  Using disulfide bond engineering to study conformational changes in the beta'260-309 coiled-coil region of Escherichia coli RNA polymerase during sigma(70) binding.

Authors:  Larry C Anthony; Alan A Dombkowski; Richard R Burgess
Journal:  J Bacteriol       Date:  2002-05       Impact factor: 3.490

5.  Interaction of RNA polymerase with forked DNA: evidence for two kinetically significant intermediates on the pathway to the final complex.

Authors:  Laura Tsujikawa; Oleg V Tsodikov; Pieter L deHaseth
Journal:  Proc Natl Acad Sci U S A       Date:  2002-03-12       Impact factor: 11.205

6.  Contributions of UP elements and the transcription factor FIS to expression from the seven rrn P1 promoters in Escherichia coli.

Authors:  C A Hirvonen; W Ross; C E Wozniak; E Marasco; J R Anthony; S E Aiyar; V H Newburn; R L Gourse
Journal:  J Bacteriol       Date:  2001-11       Impact factor: 3.490

7.  UP element-dependent transcription at the Escherichia coli rrnB P1 promoter: positional requirements and role of the RNA polymerase alpha subunit linker.

Authors:  W Meng; T Belyaeva; N J Savery; S J Busby; W E Ross; T Gaal; R L Gourse; M S Thomas
Journal:  Nucleic Acids Res       Date:  2001-10-15       Impact factor: 16.971

8.  The RNA polymerase III transcription initiation factor TFIIIB participates in two steps of promoter opening.

Authors:  G A Kassavetis; G A Letts; E P Geiduschek
Journal:  EMBO J       Date:  2001-06-01       Impact factor: 11.598

9.  Topography of lacUV5 initiation complexes.

Authors:  V Studitsky; K Brodolin; Y Liu; A Mirzabekov
Journal:  Nucleic Acids Res       Date:  2001-02-01       Impact factor: 16.971

10.  Synergistic transcription activation: a dual role for CRP in the activation of an Escherichia coli promoter depending on MalT and CRP.

Authors:  E Richet
Journal:  EMBO J       Date:  2000-10-02       Impact factor: 11.598
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