Literature DB >> 10877816

Screening of environmental DNA libraries for the presence of genes conferring lipolytic activity on Escherichia coli.

A Henne1, R A Schmitz, M Bömeke, G Gottschalk, R Daniel.   

Abstract

Environmental DNA libraries prepared from three different soil samples were screened for genes conferring lipolytic activity on Escherichia coli clones. Screening on triolein agar revealed 1 positive clone out of 730,000 clones, and screening on tributyrin agar revealed 3 positive clones out of 286,000 E. coli clones. Substrate specificity analysis revealed that one recombinant strain harbored a lipase and the other three contained esterases. The genes responsible for the lipolytic activity were identified and characterized.

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Year:  2000        PMID： 10877816      PMCID： PMC92121          DOI： 10.1128/AEM.66.7.3113-3116.2000

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  24 in total

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