| Literature DB >> 10869553 |
S Tarabykina1, M Kriajevska, D J Scott, T J Hill, D Lafitte, P J Derrick, G G Dodson, E Lukanidin, I Bronstein.
Abstract
S100A4 (Mts1) is a Ca(2+)-binding protein of the S100 family. This protein plays an important role in promoting tumor metastasis. In order to identify S100A4 interacting proteins, we have applied the yeast two-hybrid system as an in vivo approach. By screening a mouse mammary adenocarcinoma library, we have demonstrated that S100A4 forms a heterocomplex with S100A1, another member of the S100 family. The non-covalent heterodimerization was confirmed by fluorescence spectroscopy and electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry. Mutational analysis revealed that replacement of Cys(76) and/or Cys(81) of S100A4 by Ser abolishes the S100A4/S100A1 heterodimerization, but does not affect the S100A4 homodimerization in vivo.Entities:
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Year: 2000 PMID： 10869553 DOI： 10.1016/s0014-5793(00)01652-5
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124