Literature DB >> 10856773

Simple detection method for distinguishing dead and living yeast colonies.

J Kucsera1, K Yarita, K Takeo.   

Abstract

A rapid and simple assay was developed for detection of yeast colonies containing dying or dead cells. Methylene blue, phloxin B, rose bengal and trypan blue at concentrations of 5-10 micromol l(-1) were shown to stain non-viable cells in colonies of Saccharomyces cerevisiae, Schizosaccharomyces pombe, Candida albicans and Filobasidium capsuligenum without staining or affecting the viability of living cells of the colonies.

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Year:  2000        PMID： 10856773     DOI： 10.1016/s0167-7012(00)00136-6

Source DB:  PubMed          Journal:  J Microbiol Methods        ISSN： 0167-7012            Impact factor:   2.363


  21 in total

1.  Genome-wide transcription profiling of the early phase of biofilm formation by Candida albicans.

Authors:  Luis A Murillo; George Newport; Chung-Yu Lan; Stefan Habelitz; Jan Dungan; Nina M Agabian
Journal:  Eukaryot Cell       Date:  2005-09

2.  Siliques are Red1 from Arabidopsis acts as a bidirectional amino acid transporter that is crucial for the amino acid homeostasis of siliques.

Authors:  Friederike Ladwig; Mark Stahl; Uwe Ludewig; Axel A Hirner; Ulrich Z Hammes; Ruth Stadler; Klaus Harter; Wolfgang Koch
Journal:  Plant Physiol       Date:  2012-02-06       Impact factor: 8.340

3.  In vivo and in vitro anaerobic mating in Candida albicans.

Authors:  Raluca Dumitru; Dhammika H M L P Navarathna; Camile P Semighini; Christian G Elowsky; Razvan V Dumitru; Daniel Dignard; Malcolm Whiteway; Audrey L Atkin; Kenneth W Nickerson
Journal:  Eukaryot Cell       Date:  2007-01-26

4.  Characterization of cytopathic factors through genome-wide analysis of the Zika viral proteins in fission yeast.

Authors:  Ge Li; Melissa Poulsen; Csaba Fenyvuesvolgyi; Yoko Yashiroda; Minoru Yoshida; J Marc Simard; Robert C Gallo; Richard Y Zhao
Journal:  Proc Natl Acad Sci U S A       Date:  2017-01-03       Impact factor: 11.205

5.  Characterization of lapachol cytotoxicity: contribution of glutathione depletion for oxidative stress in Saccharomyces cerevisiae.

Authors:  Frederico A V Castro; Gabriel F M de Souza; Marcos D Pereira
Journal:  Folia Microbiol (Praha)       Date:  2019-06-11       Impact factor: 2.099

6.  Interleukin-18 and gamma interferon production by oral epithelial cells in response to exposure to Candida albicans or lipopolysaccharide stimulation.

Authors:  Mahmoud Rouabhia; Geneviève Ross; Nathalie Pagé; Jamila Chakir
Journal:  Infect Immun       Date:  2002-12       Impact factor: 3.441

7.  Prion-promoted phosphorylation of heterologous amyloid is coupled with ubiquitin-proteasome system inhibition and toxicity.

Authors:  Zi Yang; David E Stone; Susan W Liebman
Journal:  Mol Microbiol       Date:  2014-08-05       Impact factor: 3.501

8.  Old yeasts, young beer-The industrial relevance of yeast chronological life span.

Authors:  Ruben Wauters; Scott J Britton; Kevin J Verstrepen
Journal:  Yeast       Date:  2021-05-31       Impact factor: 3.239

9.  Cell division is essential for elimination of the yeast [PSI+] prion by guanidine hydrochloride.

Authors:  Lee J Byrne; Brian S Cox; Diana J Cole; Martin S Ridout; Byron J T Morgan; Mick F Tuite
Journal:  Proc Natl Acad Sci U S A       Date:  2007-07-02       Impact factor: 11.205

10.  A functional yeast survival screen of tumor-derived cDNA libraries designed to identify anti-apoptotic mammalian oncogenes.

Authors:  Moritz Eißmann; Bettina Schwamb; Inga Maria Melzer; Julia Moser; Dagmar Siele; Ulrike Köhl; Ralf Joachim Rieker; David Lukas Wachter; Abbas Agaimy; Esther Herpel; Peter Baumgarten; Michel Mittelbronn; Stefanie Rakel; Donat Kögel; Stefanie Böhm; Tony Gutschner; Sven Diederichs; Martin Zörnig
Journal:  PLoS One       Date:  2013-05-22       Impact factor: 3.240
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