Literature DB >> 108533

Evaluation of several enrichment procedures for the isolation of recombinant plasmid DNA.

A Rosner, R N Bastos, H Aviv.   

Abstract

A number of methods for the selective enrichment of recombinant plasmids were examined; these include alkaline phosphatase treatment of the restricted pBR322 vector, as well as a combination of this and S1 nuclease treatment of the ligated mixture of pBR322 and pCR1 plasmids or S. griseus DNA followed by D-cycloserine treatment to enrich for cells carrying recombinant molecules. The relative efficiencies of these methods were compared.

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Year:  1979        PMID： 108533     DOI： 10.1007/bf00777564

Source DB:  PubMed          Journal:  Mol Biol Rep        ISSN： 0301-4851            Impact factor:   2.316


  11 in total

1.  Reduction of possible hazards in the preparation of recombinant plasmid DNA.

Authors:  C Weissmann; W Boll
Journal:  Nature       Date:  1976-06-03       Impact factor: 49.962

2.  Gene cloning and containment properties of plasmid Col E1 and its derivatives.

Authors:  K A Armstrong; V Hershfield; D R Helinski
Journal:  Science       Date:  1977-04-08       Impact factor: 47.728

3.  Utilization of two distinct modes of replication by a hybrid plasmid constructed in vitro from separate replicons.

Authors:  K Timmis; F Cabello; S N Cohen
Journal:  Proc Natl Acad Sci U S A       Date:  1974-11       Impact factor: 11.205

4.  Nuclear satellite DNAs of yeast.

Authors:  J Retèl; R J Planta
Journal:  Biochim Biophys Acta       Date:  1972-10-27

5.  Molecular cloning of DNA fragments produced by restriction endonucleases Sa1I and BamI.

Authors:  D H Hamer; C A Thomas
Journal:  Proc Natl Acad Sci U S A       Date:  1976-05       Impact factor: 11.205

6.  Rat insulin genes: construction of plasmids containing the coding sequences.

Authors:  A Ullrich; J Shine; J Chirgwin; R Pictet; E Tischer; W J Rutter; H M Goodman
Journal:  Science       Date:  1977-06-17       Impact factor: 47.728

7.  Nonchromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA.

Authors:  S N Cohen; A C Chang; L Hsu
Journal:  Proc Natl Acad Sci U S A       Date:  1972-08       Impact factor: 11.205

8.  Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.

Authors:  F Bolivar; R L Rodriguez; P J Greene; M C Betlach; H L Heyneker; H W Boyer; J H Crosa; S Falkow
Journal:  Gene       Date:  1977       Impact factor: 3.688

9.  Kinetic and genetic analyses of D-cycloserine inhibition and resistance in Escherichia coli.

Authors:  R Curtiss; L J Charamella; C M Berg; P E Harris
Journal:  J Bacteriol       Date:  1965-11       Impact factor: 3.490

10.  The generation of a ColE1-Apr cloning vehicle which allows detection of inserted DNA.

Authors:  M So; R Gill; S Falkow
Journal:  Mol Gen Genet       Date:  1975-12-30
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  1 in total

1.  Plasmid deoxyribonucleic acid replication in Streptomyces griseus.

Authors:  Y Xue; Z Zhuang; Y Zhu; Y Xu; K Dong
Journal:  J Bacteriol       Date:  1981-04       Impact factor: 3.490
  1 in total

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