The differentiation of cytotoxic T lymphocytes in vitro: an ultrastructural study.

T killer cells were identified morphologically in two different culture systems. The first system allowed identification by comparing under identical culture conditions two types of stimulation, one leading to cytotoxic activity levels that were very high (H-2 stimulation) and the other to levels which were practically nil (M-locus stimulation). A second system allowed identification by the use of a collaborative system in which relatively pure precursors of T killer cells were mixed with precursors of T amplifier cells from which they were distinguishable by their Thy-l antigen. In this latter case, surface labelling techniques had to be used. As in the in vivo situation, T killer cells were medium-sized pale cells with evidence of a well-developed secretory and motile apparatus. No evidence of highly active protein synthesis was found. Other T cells were dark and mainly derived from the T amplifier population, again consistent with in vivo findings. There was considerable background proliferation, even in the absence of any added antigen, and this included the formation of some well-developed plasma cells.