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Literature DB >> 1078978

Isolation and identification of 1,25-dihydroxyvitamin D2.

Abstract

The chemical synthesis of [3alpha-3H]vitamin D2 of high specific activity has been described. With the use of this radioactive material, the existence of a polar metabolite believed to be the active form of vitamin D2 in the rat and chick has been demonstrated. It has been isolated in pure form from an in vitro chick kidney mitochondrial system and identified as 1,25-dihydroxyvitamin D2 by means of mass spectrometry, ultraviolet absorption spectrophotometry, and specific derivative synthesis. Its antirachitic activity equals that of 1,25-dihydroxyvitamin D3 in the rat.

Entities: Chemical Species

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Year: 1975 PMID： 1078978 DOI： 10.1021/bi00677a025

Source DB: PubMed Journal: Biochemistry ISSN： 0006-2960 Impact factor: 3.162

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Cited

7 in total

Review 1. History of the discovery of vitamin D and its active metabolites.

Authors: Hector F Deluca
Journal: Bonekey Rep Date: 2014-01-08

2. The effect of hepatectomy on the synthesis of 25-hydroxyvitamin D3.

Authors: E B Olson; J C Knutson; M H Bhattacharyya; H F DeLuca
Journal: J Clin Invest Date: 1976-05 Impact factor: 14.808

3. A newly developed and validated LC-MS/MS method for measuring 7-dehydrocholesterol (7DHC) concentration in human skin: a tool for vitamin D photobiology research.

Authors: Oktawia Borecka; Lesley E Rhodes; Ann R Webb; John J Dutton; William D Fraser
Journal: Photochem Photobiol Sci Date: 2022-07-29 Impact factor: 4.328

4. 1 alpha,24(S)-dihydroxyvitamin D2: a biologically active product of 1 alpha-hydroxyvitamin D2 made in the human hepatoma, Hep3B.

Authors: S Strugnell; V Byford; H L Makin; R M Moriarty; R Gilardi; L W LeVan; J C Knutson; C W Bishop; G Jones
Journal: Biochem J Date: 1995-08-15 Impact factor: 3.857

Isolation and identification of 1,25-dihydroxyvitamin D2.

Review 1. History of the discovery of vitamin D and its active metabolites.

2. The effect of hepatectomy on the synthesis of 25-hydroxyvitamin D3.

3. A newly developed and validated LC-MS/MS method for measuring 7-dehydrocholesterol (7DHC) concentration in human skin: a tool for vitamin D photobiology research.

4. 1 alpha,24(S)-dihydroxyvitamin D2: a biologically active product of 1 alpha-hydroxyvitamin D2 made in the human hepatoma, Hep3B.

5. Discrimination in the metabolism of orally dosed ergocalciferol and cholecalciferol by the pig, rat and chick.

Review 6. 100 YEARS OF VITAMIN D: Historical aspects of vitamin D.

Review 7. A new look at the most successful prodrugs for active vitamin D (D hormone): alfacalcidol and doxercalciferol.