Literature DB >> 10788418

Production and characterization of monoclonal antibodies against vegetative cells of Bacillus cereus.

N Charni1, C Perissol, J Le Petit, N Rugani.   

Abstract

Two monoclonal antibodies (MAbs) against Bacillus cereus were produced. The MAbs (8D3 and 9B7) were selected by enzyme-linked immunosorbent assay for their reactivity with B. cereus vegetative cells. They reacted with B. cereus vegetative cells while failing to recognize B. cereus spores. Immunoblotting revealed that MAb 8D3 recognized a 22-kDa antigen, while MAb 9B7 recognized two antigens with molecular masses of approximately 58 and 62 kDa. The use of MAbs 8D3 and 9B7 in combination to develop an immunological method for the detection of B. cereus vegetative cells in foods was investigated.

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Year:  2000        PMID： 10788418      PMCID： PMC101491          DOI： 10.1128/AEM.66.5.2278-2281.2000

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  21 in total

Review 1.  Foodborne disease due to Bacillus and Clostridium species.

Authors:  B M Lund
Journal:  Lancet       Date:  1990-10-20       Impact factor: 79.321

2.  Psychrotrophic strains of Bacillus cereus producing enterotoxin.

Authors:  P van Netten; A van De Moosdijk; P van Hoensel; D A Mossel; I Perales
Journal:  J Appl Bacteriol       Date:  1990-07

3.  A rapid PCR-based DNA test for enterotoxic Bacillus cereus.

Authors:  V Mäntynen; K Lindström
Journal:  Appl Environ Microbiol       Date:  1998-05       Impact factor: 4.792

4.  Preparation of monoclonal antibodies: strategies and procedures.

Authors:  G Galfrè; C Milstein
Journal:  Methods Enzymol       Date:  1981       Impact factor: 1.600

5.  Comparative analysis of Bacillus anthracis, Bacillus cereus, and related species on the basis of reverse transcriptase sequencing of 16S rRNA.

Authors:  C Ash; J A Farrow; M Dorsch; E Stackebrandt; M D Collins
Journal:  Int J Syst Bacteriol       Date:  1991-07

6.  Toxin production by Bacillus cereus dairy isolates in milk at low temperatures.

Authors:  A Christiansson; A S Naidu; I Nilsson; T Wadström; H E Pettersson
Journal:  Appl Environ Microbiol       Date:  1989-10       Impact factor: 4.792

7.  Rapid screening test for enterotoxin-producing Bacillus cereus.

Authors:  S G Jackson
Journal:  J Clin Microbiol       Date:  1993-04       Impact factor: 5.948

8.  Differentiation of dairy strains of the Bacillus cereus group by phage typing, minimum growth temperature, and fatty acid analysis.

Authors:  O M Väisänen; N J Mwaisumo; M S Salkinoja-Salonen
Journal:  J Appl Bacteriol       Date:  1991-04

9.  Enumeration of Bacillus cereus in foods.

Authors:  D A Mossel; M J Koopman; E Jongerius
Journal:  Appl Microbiol       Date:  1967-05

10.  Enumeration and identification of Bacillus cereus in foods. I. 24-hour presumptive test medium.

Authors:  H U Kim; J M Goepfert
Journal:  Appl Microbiol       Date:  1971-10
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