| Literature DB >> 10780388 |
T Hohsaka1, Y Ashizuka, M Sisido.
Abstract
A novel method of the in vitro incorporation of two nonnatural amino acids into proteins through extension of the genetic code was developed. The streptavidin mRNA containing AGGU and CGGG, and chemically aminoacylated tRNA(ACCU) and tRNA(CCCG) were prepared, then they were added into E. coli in vitro protein synthesizing system. As a result, two nonnatural amino acids were successfully incorporated into desired sites of streptavidin.Entities:
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Year: 1999 PMID： 10780388 DOI： 10.1093/nass/42.1.79
Source DB: PubMed Journal: Nucleic Acids Symp Ser ISSN： 0261-3166