| Literature DB >> 10779681 |
H Kawagishi1, H Suzuki, H Watanabe, H Nakamura, T Sekiguchi, T Murata, T Usui, K Sugiyama, H Suganuma, T Inakuma, K Ito, Y Hashimoto, M Ohnishi-Kameyama, T Nagata.
Abstract
In an experiment in which rats were allowed free access to food and water, the rats did not eat the diet containing a mushroom Pleurotus ostreatus even if they were emaciated. A P. ostreatus lectin (POL) was isolated from the mushroom as the food intake-suppression principle. In hemagglutination inhibition assays, Me-alphaGalNAc was the most potent inhibitor among the monosaccharides tested. Among all the sugars tested, 2'-fucosyllactose (Fucalpha1-->2Galbeta1-->4Glc) was the strongest inhibitor and its inhibitory potency was five times greater than that of Me-alphaGalNAc. POL exhibited a binding ability to bovine submaxillary mucin (BSM) and asialo-BSM and the other glycoproteins were inert to the binding. The food intake-suppressing activity of POL was dependent on the dose. The diet containing 0.1% POL caused a 50% decrease in the food intake of rats against the control.Entities:
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Year: 2000 PMID： 10779681 DOI： 10.1016/s0304-4165(00)00027-1
Source DB: PubMed Journal: Biochim Biophys Acta ISSN： 0006-3002