| Literature DB >> 10779577 |
H Ikadai1, C R Osorio, X Xuan, I Igarashi, T Kanemaru, H Nagasawa, K Fujisaki, N Suzuki, T Mikami.
Abstract
The 48-kDa Babesia caballi merozoite rhoptry protein was expressed using a pGEX4T expression vector in Escherichia coli as glutathione S-transferase fusion protein (GST-BC48), and the expressed GST-BC48 was used in an ELISA to detect specific antibodies in serum samples. No cross-reaction was observed with sera from horses experimentally infected with Babesia equi. GST-BC48 ELISA was a highly sensitive and specific test when compared with the CFT. A total of 209 horse sera obtained from Central Mongolia were examined with the GST-BC48 ELISA and 46.4% (97/209) were found to be seropositive for B. caballi, suggesting that the GST-BC48 ELISA can be successfully used for both quarantine and epidemiological studies.Entities:
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Year: 2000 PMID： 10779577 DOI： 10.1016/s0020-7519(00)00008-4
Source DB: PubMed Journal: Int J Parasitol ISSN： 0020-7519 Impact factor: 3.981