Literature DB >> 14715570

Molecular cloning of a Babesia caballi gene encoding the 134-kilodalton protein and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay.

Yoh Tamaki1, Haruyuki Hirata, Noriyuki Takabatake, Sabine Bork, Naoaki Yokoyama, Xuenan Xuan, Kozo Fujisaki, Ikuo Igarashi.   

Abstract

A Babesia caballi gene encoding the 134-kDa (BC134) protein was immunoscreened with B. caballi-infected horse serum. An enzyme-linked immunosorbent assay (ELISA) using recombinant BC134 protein could effectively differentiate B. caballi-infected horse sera from Babesia equi-infected or noninfected control horse sera. These results suggest that the recombinant BC134 protein is a potential diagnostic antigen in the detection of B. caballi infection.

Entities:  

Mesh：

Substances：

Year:  2004        PMID： 14715570      PMCID： PMC321337          DOI： 10.1128/cdli.11.1.211-215.2004

Source DB:  PubMed          Journal:  Clin Diagn Lab Immunol        ISSN： 1071-412X


  24 in total

1.  Cellular localization of Babesia bovis merozoite rhoptry-associated protein 1 and its erythrocyte-binding activity.

Authors:  Naoaki Yokoyama; Boonchit Suthisak; Haruyuki Hirata; Tomohide Matsuo; Noboru Inoue; Chihiro Sugimoto; Ikuo Igarashi
Journal:  Infect Immun       Date:  2002-10       Impact factor: 3.441

2.  Isolation of Plasmodium berghei (malaria) parasites by ammonium chloride lysis of infected erythrocytes.

Authors:  W J Martin; J Finerty; A Rosenthal
Journal:  Nat New Biol       Date:  1971-10-27

3.  Biology of equine piroplasmosis.

Authors:  A A Holbrook
Journal:  J Am Vet Med Assoc       Date:  1969-07-15       Impact factor: 1.936

4.  Characterisation of the gene encoding a protective antigen from Babesia microti identified it as eta subunit of chaperonin containing T-complex protein 1.

Authors:  M Nishisaka; N Yokoyama; X Xuan; N Inoue; H Nagasawa; K Fujisaki; T Mikami; I Igarashi
Journal:  Int J Parasitol       Date:  2001-12       Impact factor: 3.981

5.  Cloning and expression of a 48-kilodalton Babesia caballi merozoite rhoptry protein and potential use of the recombinant antigen in an enzyme-linked immunosorbent assay.

Authors:  H Ikadai; X Xuan; I Igarashi; S Tanaka; T Kanemaru; H Nagasawa; K Fujisaki; N Suzuki; T Mikami
Journal:  J Clin Microbiol       Date:  1999-11       Impact factor: 5.948

6.  Cloning of a truncated Babesia equi gene encoding an 82-kilodalton protein and its potential use in an enzyme-linked immunosorbent assay.

Authors:  Haruyuki Hirata; Hiromi Ikadai; Naoaki Yokoyama; Xuenan Xuan; Kozo Fujisaki; Naoyoshi Suzuki; Takeshi Mikami; Ikuo Igarashi
Journal:  J Clin Microbiol       Date:  2002-04       Impact factor: 5.948

7.  Molecular characterization of the amplified carboxylesterase gene associated with organophosphorus insecticide resistance in the brown planthopper, Nilaparvata lugens.

Authors:  G J Small; J Hemingway
Journal:  Insect Mol Biol       Date:  2000-12       Impact factor: 3.585

8.  Identification of a specific antigenic region of the P82 protein of Babesia equi and its potential use in serodiagnosis.

Authors:  Haruyuki Hirata; Xuenan Xuan; Naoaki Yokoyama; Yoshifumi Nishikawa; Kozo Fujisaki; Naoyoshi Suzuki; Ikuo Igarashi
Journal:  J Clin Microbiol       Date:  2003-02       Impact factor: 5.948

9.  Species-specific serodiagnosis of equine piroplasma infections by means of complement fixation test (CFT), immunofluorescence (IIF), and enzyme-linked immunosorbent assay (ELISA).

Authors:  G Weiland
Journal:  Vet Parasitol       Date:  1986-03       Impact factor: 2.738

Review 10.  [Piroplasmas of horses--impact on the international horse trade].

Authors:  K T Friedhoff
Journal:  Berl Munch Tierarztl Wochenschr       Date:  1982-10-01       Impact factor: 0.328
View more
  1 in total

1.  Cloning of a novel Babesia equi gene encoding a 158-kilodalton protein useful for serological diagnosis.

Authors:  Haruyuki Hirata; Naoaki Yokoyama; Xuenan Xuan; Kozo Fujisaki; Naoyoshi Suzuki; Ikuo Igarashi
Journal:  Clin Diagn Lab Immunol       Date:  2005-02
  1 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.