| Literature DB >> 10775831 |
V Silei1, C Fabrizi, G Venturini, F Tagliavini, M Salmona, O Bugiani, G M Lauro.
Abstract
The fluorescent calcium-sensitive indicators, such as the Calcium Green-1, allow one to detect small calcium transients at low indicator concentrations. The protocol reported here is a rapid and sensitive method that facilitates the measurement of intracellular free-calcium in cell suspensions. Using this assay, we were able to detect and quantify the variations in intracellular calcium concentration during microglial cell activation induced by the fragment peptides beta25-35 and PrP106-126.Entities:
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Year: 2000 PMID： 10775831 DOI： 10.1016/s1385-299x(00)00003-9
Source DB: PubMed Journal: Brain Res Brain Res Protoc ISSN： 1385-299X